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Heterologous Expression of Membrane Proteins - Methods and Protocols (Hardcover, 3rd ed. 2022)
Loot Price: R6,300
Discovery Miles 63 000
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Heterologous Expression of Membrane Proteins - Methods and Protocols (Hardcover, 3rd ed. 2022)
Series: Methods in Molecular Biology, 2507
Expected to ship within 12 - 17 working days
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Total price: R6,320
Discovery Miles: 63 200
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This detailed volume explores protocols for the production of
membrane proteins in a panel of heterologous organisms for
structural studies. Beginning with techniques using E. coli as a
host for the overproduction and purification of membrane proteins,
the book continues with chapters covering mammalian membrane
protein production in yeast, insect cells, mammalian cells, as well
as using virus like particles and acellular systems. Additionally,
new detergents and alternatives to detergents allowing membrane
protein purification for structural analyses are described. The
book closes with a chapter exploring the use of microscale
thermophoresis (MST) to evaluate the binding activity of
heterologously expressed proteins directly in crude membrane
extracts. Written for the highly successful Methods in Molecular
Biology series, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Authoritative and
up-to-date, Heterologous Expression of Membrane Proteins: Methods
and Protocols, Third Edition serves as an ideal guide for
scientists aiming to produce and purify functional recombinant
membrane proteins for structural studies.
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