Fibrosis Research - Methods and Protocols (Paperback, Softcover reprint of hardcover 1st ed. 2005)

Leading investigators review the highlights of current fibrosis research and the experimental methodologies used uncover the mechanisms that drive it. In their discussion of research methodologies utilizing cultured cells to model various aspects of the fibrotic response in vitro, the authors describe the isolation, characterization, and propagation of mesenchymal cells, and highlight the similarities and differences between methods that are appropriate for different types of fibroblasts. Approaches for studying collagen gene regulation and TGF-b production are also discussed, along with experimental methodologies utilizing animal models to study the pathogenesis of fibrosis. The protocols follow the successful Methods in Molecular Medicine (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

General

Imprint:	HumanaPress
Country of origin:	United States
Series:	Methods in Molecular Medicine, 117
Release date:	November 2010
First published:	2005
Editors:	John Varga • David A. Brenner • Sem H. Phan
Dimensions:	229 x 152 x 25mm (L x W x T)
Format:	Paperback
Pages:	392
Edition:	Softcover reprint of hardcover 1st ed. 2005
ISBN-13:	978-1-61737-592-7
Categories:	Books > Medicine > Clinical & internal medicine > Respiratory medicine
LSN:	1-61737-592-6
Barcode:	9781617375927

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