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Books > Professional & Technical > Environmental engineering & technology > Sanitary & municipal engineering > Water supply & treatment
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Use of Novel Techniques to Quantify Phenotypes in Biological Treatment Process (Paperback, Illustrated Ed)
Loot Price: R3,387
Discovery Miles 33 870
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Use of Novel Techniques to Quantify Phenotypes in Biological Treatment Process (Paperback, Illustrated Ed)
Series: WERF Research Report Series
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This research attempts to evaluate nitrification treatment
performance in combined carbon/nitrogen municipal wastewater
reactors using traditional physical/chemical methods and modern
molecular techniques. Bench scale activated sludge reactors were
operated at different SRTs under varying DO levels and temperatures
over a 21-month period. Real-time PCR assays were used to determine
cell concentrations of total bacterial 16S rDNA, a gross measure of
biomass content, the amoA gene, a measure of ammonia-oxidizing
bacteria (AOB), and the Nitrospira 16S rDNA gene, a measure of
nitrite-oxidizing bacteria (NOB). As expected, gravimetric biomass
and total bacterial 16S rDNA levels increased with increasing SRT.
Ammonia oxidation rates and N. oligotropha-type AOB concentrations
did not follow similar trends with respect to changes in SRT,
temperature, and DO nor were they highly correlated. The
concentration of available nitrite and SRT were positively
correlated with Nitrospira cell densities, while DO concentration
and temperature were negatively correlated with NOB levels. The
percentage of the total population comprised of AOB and NOB
obtained with the real-time PCR assays were compared to predicted
values estimated from design equations using typical kinetic
parameters. While the percentages of NOB measured using the
real-time PCR assay corresponded very well with the predicted
values, the measured percentages of AOB were much lower than those
estimated from the design equations, suggesting that N.
oligotropha-type AOB were not the dominant ammonia-oxidizing
species in these reactors. This publication can be purchased and
downloaded via Pay Per View on Water Intelligence Online - click on
the Pay Per View icon below
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