In the last few years, significant breakthroughs in
transcription research expanded our appreciation for the complexity
of molecular controls on gene expression in mammalian cells. In
Transcription Factors: Methods and Protocols, experts in the field
describe state-of-the-art approaches that investigators can use to
probe critical mechanisms underlying transcription factor
nuclear-cytoplasmic trafficking as well as to assess the functional
impact of post-translational modifications on transcription factor
function. The chapters are written by prominent scientists, many of
whom developed these methods, and highlight protocols that focus on
specific transcription factor family members with particular
relevance to human disease. Composed in the highly successful
Methods in Molecular Biology(TM) series format, each chapter
contains a brief introduction, step-by-step methods, a list of
necessary materials, and a Notes section which shares tips on
troubleshooting and avoiding known pitfalls.
Comprehensive and current, Transcription Factors: Methods and
Protocols compiles the latest techniques for elucidating controls
on transcription factor intracellular localization and activity,
and consequently is unlike any other methods-based text on
transcriptional regulation today.
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