Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is
a comprehensive review of nuclear transfer technology in
vertebrates, aimed at reprogramming differentiated nuclei and
effecting targeted gene transfer. The emphasis here is on providing
readily reproducible techniques for the gene- tion of cloned
embryos and animals in a number of key research and commercially
important vertebrates. Additional chapters provide alternative
cutting-edge methods for nuclear transfer, such as zona-free
nuclear transfer and serial nuclear transfer. Of immense practical
benefit are descriptions of procedures associated with cloning,
such as in vitro maturation of oocytes, activation and culture of
cloned embryos, maintenance of pregnancy, and neonatal care of
clones. Nuclear Transfer Protocols: Cell Reprogramming and
Transgenesis also provides an understanding of the factors involved
in nuclear reprogramming, which is imperative for the success of
cloning. A section dealing with such cloning-related issues as
aging and normality of clones is also included making this an
essential comprehensive handbook for research and commercial labo-
tories involved in, or intending to work on, nuclear transfer. The
volume will prove beneficial to molecular biologists, stem cell
biologists, clinicians, biotechnologists, students, veterinarians,
and animal care technicians involved with reprogramming, nuclear
transfer, and transgenesis.
General
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