The tissue culture approach to the study of membrane properties of
excitable cells has progressed beyond the technical problems of
culture methodology. Recent developments have fostered substantive
contributions in research con cerned with the physiology,
pharmacology, and biophysics of cell membranes in tissue culture.
The scope of this volume is related to the application of tissue
culture methodology to developmental processes and cellular
mechanisms of electrical and chemical excitability. The major
emphasis will be on the body of new biological information made
available by the analytic possibilities inherent in the tissue
culture systems. Naturally occurring preparations of excitable
cells are frequently of suf ficient morphological complexity to
compromise the analysis of the data obtained from them. Some of the
limitations associated with dissected prepa rations have to do with
the direct visualization of and access to the cell(s) in question
and maintenance of steady-state conditions for prolonged periods of
time. Since preparations in tissue culture can circumvent these
problems, it is feasible to analyze the properties of identifiable
cells, grown either singly or in prescribed geometries, as well as
to follow the development of cellular inter actions. A crucial
consideration in the use of cultured preparations is that they must
faithfully capture the phenomenon of interest to the investigator.
This and other potential limitations on the methodology are of
necessary concern in the present volume.
General
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