The objective of this project was to develop and evaluate a
practical technique to indicate the probability of the presence of
bacterial pathogens in receiving waters. A "practical" method was
defined as one that would limit the use of defined culture-based
microbiological methods, and would be based on: (1) validating
indicator organisms that predicted the presence of pathogens, or
(2) detection of pathogens by polymerase chain reaction (PCR)-based
assays. The study also assessed the utility of PCR-based technology
for bacterial pathogen detection with respect to technology
transfer to a wider range of water and wastewater facilities.
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