The germline is unique in mammals as it is the only cell lineage
that undergoes mitosis, meiosis, and differentiation, making these
cells amenable to in-depth genomic and mechanistic studies. In
Germline Development: Methods and Protocols, expert researchers
provide a wide-ranging collection of techniques that can be applied
to the study of both the male and female germline. As the
techniques used for isolating and studying male and female germ
cells are slightly dissimilar, the different technologies are
grouped under two sections in this volume, namely, male germline
and female germline. This collection brings the field of germline
developmental studies together such that readers can find, in a
single volume, techniques that allow the isolation of gonads with
germ cells at different stages of development, the study of the
genomics and epigenomics of germ cells, and an examination of the
role of single genes in the developmental potential and function of
germ cells. Written in the highly successful Methods in Molecular
Biology (TM) series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and the avoidance of known pitfalls.
Comprehensive and cutting-edge, Germline Development: Methods and
Protocols serves as a comprehensive text to individuals,
particularly researchers in reproductive and developmental biology,
who are interested in studying cells of the germ lineage.
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