CRISPR Gene Editing - Methods and Protocols (Hardcover, 1st ed. 2019)

This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPR gene editing field. Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.

General

Imprint:	HumanaPress
Country of origin:	United States
Series:	Methods in Molecular Biology, 1961
Release date:	March 2019
First published:	2019
Editors:	Yonglun Luo
Dimensions:	254 x 178 x 29mm (L x W x T)
Format:	Hardcover
Pages:	362
Edition:	1st ed. 2019
ISBN-13:	978-1-4939-9169-3
Categories:	Books > Medicine > Pre-clinical medicine: basic sciences > Medical genetics Books > Professional & Technical > Biochemical engineering > Biotechnology > Genetic engineering
LSN:	1-4939-9169-8
Barcode:	9781493991693

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