Human beings have on the order of 100,000 different genes encoding the molecules needed to build and operate the human body; defects in any one of them can lead to disastrous consequences. There are an estimated 4,000 genetic diseases, which can be every bit as devastating as the diseases caused by bacteria or viruses, and in one way they are much worse: we pass them on to our children, generation after generation after generation. The New Healers is the story of the devastation these diseases cause, and the scientific researchers and doctors who struggle to combat them. Science and medicine have provided us with clues to the treatment of a few genetic diseases, although by their very nature they have never been considered curable. But, as William R. Clark shows, that is about to change through one of the most profound revolutions in modern medicine: gene therapy, a branch of the new field of molecular medicine. Clark takes us to the laboratories which have been able to isolate human genes, to make billions of copies of them, and to reintroduce healthy genes into unfortunate individuals who have inherited damaged or functionless genes. He also shows us how this same technology, turned around on itself, can also be used to deliberately introduce "bad" genes to attack and destroy unwanted cells, such as cancer cells or those infected with the AIDS virus. Molecular medicine will be a major part of our lives in the new millennium. The New Healers outlines the powerful and compelling logic behind molecular medicine: that everything we know about molecular biology tells us that it can work, and that it will work. Clark introduces us to the scientists working now to map out the entire human genome, easily the medical equivalent of going to the moon, taking human beings to a completely new level of understanding of our biological selves. Clark also helps us to begin thinking about how we will manage that understanding, and how we will use the information we gain. The New Healers is a clear and compelling introduction to this important new frontier of human medicine, outlining for readers all the basic elements of molecular biology necessary to understand molecular medicine, and illustrating the fascinating stories of those doctors and patients already a part of this exciting future -- a future as full of promise as anything we have witnessed in this past century of remarkable progress.

This text provides an investigation into how the Human Genome Project (HGP) is likely to affect future generations. It examines the implications these effects hold for evaluating HGP and other research efforts like it.

Electroporation is one of the most widespread techniques used in modem molecular genetics. It is most commonly used to introduce DNA into cells for investigations of gene structure and function, and in this regard, electroporation is both highly versatile, being effective with nearly all species and cell types, and highly efficient. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. However, exposure of cells to brief, hi- intensity electric fields has found broad application in other aspects of biological research, and is now routinely used to introduce other types of biological and analytic molecules into cells, to induce cell-cell fusion, and to transfer DNA directly between different species. The first seven chapters of Electroporation Protocols for Micro organisms describe the underlying theory of electroporation, the com mercially available instrumentation, and a number of specialized electroporation applications, such as cDNA library construction and interspecies DNA electrotransfer. Each of the remaining chapters pre sents a well developed method for electrotransformation of a particular bacterial, fungal, or protist species. These chapters also serve to intro duce those new to the field the important research questions that are currently being addressed with particular organisms, highlighting both the major advantages and limitations of each species as a model organ ism, and explaining the roles that electroporation has played in the development of the molecular genetic systems currently in use."

Recombinant DNA techniques have revolutionized the isolation and production of antibodies in recent years. This has resulted in rapid changes in how to handle antibodies for research. This new Practical Approach volume responds to this change by assembling, in one volume, protocols which allow the researcher to isolate a new antibody, analyse its properties, format the right antibody molecule or fragment, and produce it in usable quantities. The book is divided into two parts: the first describes the generation and analysis of antibodies and the second covers engineering and production. This timely book will be of interest to those involved in both clinical and molecular biological applications of antibodies.

Gene/biotechnology and the broad use of genetically modified organisms is rapidly developing into a commercial enterprise. In spite of the pace of scientific progress in this field, a partly emotional debate is still being carried on by the general public and on the part of the consumer about the potential risks linked to this new technology. Especially when genetically modified organisms (GMOs) are tested in the open field, the question of "safety" is controversially discussed among different groups in society. Environmentalists are worried about unforeseeable dangers inherent in the release of a living organism, assuming that special risks are linked to organisms which have been modified by molecular biologists using tools developed in the laboratory. Frequently quoted potential hazards are the phenomena of "gene escape" and "genetic pollution," meaning the very interesting question of natural (horizontal) gene transfer. This phenomenon of horizontal gene transfer is in fact well known to scientists studying prokaryotes. Many prokaryotes are able to exchange DNA readily between individuals of different species or are even able to take up DNA from the extracellular environment by the process of genetic transformation, which has already been known for more than 65 years. For eukaryotes, however, the evidence of natural horizontal gene transfer is very limited.

Twenty-three papers review recent advances in experimental studies on microorganisms, plants and animals. They are taken from a symposium organized at Cologne University, in April 1983 by the Committee on Genetic Experimentation (COGENE), a scientific committee of the International Council of Scientific Unions.

With each species lost to the mass extinction crisis, the natural economy of the nation and of the world is greatly diminished. An endangered animal may hold the cure for cancer; a threatened plant could someday become a major food crop; and even bacteria often provide novel molecular structures in polymer science. As the rainforest is destroyed and habitats are degraded, conservationists are now urgently searching for dramatic new ways to save these economic resources.
In this provocative and important book, Joseph Henry Vogel details one potential solution that has met with increasing interest and popularity: the privatization of genetic information. Vogel cogently makes the case that the world should abandon the doctrine of "the common heritage of mankind" and create private property rights over genes. Landowners, once vested with the genetic resources on their land, will have a newfound financial incentive to protect what they now already control.
Genes for Sale provides an overview of the many complexities inherent in implementing a viable conservation policy. Vogel elaborates both technical issues like the construction of a "gargantuan database" of landtitles and biological inventories, and political issues like legal reform. Clearly written, engaging, and timely, Genes for Sale provides students, scientists, and policy makers alike with the ideal introduction to an exciting and controversial new approach to saving our precious living heritage.

It is now twenty years since Cohen and Boyer's first steps into DNA cloning. In the time since then, there has been an ever increasing acc- eration in the development and application of the cloning methodology. With the recent development of the polymerase chain reaction, a second generation of the technology has been born, enabling the isolation of DNA (and in particular, genes) with little more information than the p- tial knowledge of the sequence. In fact, DNA sequencing is now so advanced that it can almost be carried out on the industrial scale. As a consequence of these advances, it now appears feasible to sequence whole genomes, including one the size of the human. What are we going to do with this information? The future of basic molecular biology must lie in the ability to analyze DNA (and especially the genes within it) starting at the DNA level. It is for these problems that Protocols for Gene Analysis attempts to offer solutions. So you have a piece of DNA, possibly a gene--what do you do next? The first section of this book contains a number of "basic" te- niques that are required for further manipulation of the DNA. This s- tion is not intended to be a comprehensive collection of methods, but merely to serve as an up-to-date set of techniques. I refer you to other volumes in the Methods Molecular Biology series for further rec- binant DNA techniques.

We are poised at the doorway to a future which could surpass the Industrial Revolution in its impact on the world. We are beginning to scrutinize genes in order to read the very history of evolution, and to alter plants and animals in ways undreamed of only a few years ago. As the tools of science have become more sophisticated, scientists have been able to delve deeply into the inner recesses of cells. The fruit of their labor, the new biology, promises us an unprecedented understanding of genes, offering an illuminating view into the most intimate operations of living things from microbes to humans - and with that, the potential to gain increasing control over life itself. Our gene future will soon present us with a cornucopia of products by drawing upon a variety of organisms - plants, animals, and even human cells - that will influence the lives and health of us all. As Thomas Lee, a biologist and author of the acclaimed The Human Genome Project: Cracking the Genetic Code of Life, so vividly shows, the new biology is already beginning to make its mark on our lives. Every week newspapers announce that scientists have found the gene for a disease such as cystic fibrosis or a particular form of cancer, or have seized on a gene therapy to try to combat it. The controversy over using DNA fingerprinting as admissible evidence in court has sparked public concern. The injecting of experimental genes into humans and animals has triggered recent debates. Soon genetically engineered tomatoes and other "transgenic" vegetables will be available on the shelves of our local grocers. As Lee so wisely and eloquently cautions, there may be perils along this pathway as well as miraculous discoveries. Do dangers lurk in this new technological approach to nature? May we unwittingly be doing irreparable harm to individuals, not to mention the biosphere? This perceptive author even-handedly assesses the controversies surrounding the perils that may await us as molecular science m

The 5th edition of this successful Glossary has been completely revised, updated and supplemented by up-to-date terms used in genetic engineering and molecular genetics. Where necessary a short essay explaining an entry in more detail is added to the stated definition. Wherever possible, the author of an entry is mentioned and the respective publication cited. Cross references ease the orientation within the glossary.
"This excellent textbook should serve seasoned scientists as a feast for the mind and as a valuable work for graduate students. It is a true bargain..."(Quarterly Review of Biology) "By the very fact that this Glossary is now in its fifth edition, one can be assured of its usefulness... Highly recommended." (Australasian)

Das erste Laborhandbuch der Humangenetik. Jedes Kapitel behandelt eine Praxiseinheit. Die Themen reichen von den Grundlagen des menschlichen Erbgutes uber die Genetik von Krankheiten, webbasierte Ressourcen, Bioinformatik und Individualmedizin bis hin zur genetischen Beratung.

Covers the basic computer analyses used for new DNA sequences and attempts to provide the researcher with the necessary background in order to understand and use efficiently these programs.

Presenting all preclinical and clinical information available on genetically engineered toxins, this unique, single-source reference provides the most up-to-date methods and practical examples for conducting clinical studies in toxin molecular biology.;Reviewing difficult problems and their solutions, Genetically Engineered Toxins discusses techniques for clo;ning, expressing, and purifying recombinant toxins and genetically modified recombinant toxins; documents structure-function relationships in toxins, including comparative information; supplies theory and illustrations of chimeric toxins; delineates the preclinical assessments of new reagents; and summarizes approaches to drug design.;With over 1100 literature citations, Genetically Engineered Toxins is an invaluable resource for biochemists, molecular biologists, biotechnologists, pharmacologists, toxicologists, X-ray crystallographers, enzymologists, oncologists, hematologists, immunologists, rheumatologists, botanists, and graduate-level students in molecular biology, biotechnology, and clinical oncology courses.

'This co-authored book explores how advances in cell biology, CRISPR gene editing and bioengineering might be used to make a live dragon. The result is a gloriously tongue-in-check scientific epic ... How to Build a Dragon or Die Trying is deliberately flamboyant and outrageous. ItaEURO (TM)s also funny and smart. Far from a how-to guide for neaEURO (TM)er-do-wells to weaponize reptiles, it is designed to spark healthy curiosity in anyone who enjoys a ripping good science read.'NatureWhat if you could have your own real dragon? While that might seem like just a fantasy, today cutting-edge science has brought us to the point where it might really be possible. This book looks into the possibilities of making living, fire-breathing dragons. The world has been fascinated with dragons for thousands of years. Fictional dragons still have a firm place in pop culture, such as Smaug from The Hobbit as well as the dragons in Game of Thrones and in the How to Train Your Dragon movies. This new book discusses using powerful technologies such as CRISPR gene editing, stem cells, and bioengineering to make real dragons. It also goes through what useful information we can learn from animals such as Pteranodons and amazing present-day creatures in our quest to build actual dragons. The book goes on to discuss the possibility of building other mythical creatures such as unicorns and mermaids. Overall, How to Build A Dragon is also meant as a satirical look at cutting-edge science, and it pokes fun at science hype. Anyone who is interested in dragons or cutting-edge science will enjoy this book! It is written in a humorous, approachable way making science fun and easy to understand, including for young adults.The author is well-known scientist Paul Knoepfler who is familiar to the public for his science, his blog The Niche, and his frequent contributions to lay stories on new science concepts such as stem cells and CRISPR. He also is known for his TED talk on designer babies with more than 1.3 million views, and his two books - . The co-author, his daughter Julie Knoepfler, is a high school student interested in science and writing. She has her own blog on literary and film analysis, and enjoys taking a humorous look at culture through writing.

'A masterpiece of poignant brilliance . . . heartbreaking' Guardian Charlie Gordon, a floor sweeper born with an unusually low IQ, has been chosen as the perfect subject for an experimental surgery that doctors hope will increase his intelligence - a procedure that has been highly successful when tested on a lab mouse named Algernon. All Charlie wants is to be smart and have friends, but the treatement turns him into a genius. Then Algernon begins to fade. What will become of Charlie?

With ever-advancing scientific understanding and technological capabilities, humanity stands on the brink of the potential next stage of evolution: evolution engineered by us. Nanotechnology, biotechnology, information technology and cognitive science offer the possibility to enhance human performance, lengthen life-span and reshape our inherited physical, cognitive and emotional identities. But with this promise come huge risks, complex choices and fundamental ethical questions: about evolution; about what it is to be human; and about control over, and the distribution of benefits from, new technology. Written by a range of experts in science, technology, bioethics and social science, Unnatural Selection examines the range of technological innovations offering lives that purport to be longer, stronger, smarter and happier, and asks whether their introduction is likely to lead to more fulfilled individuals and a fairer world. The breadth of approaches and perspectives make important reading for anyone who cares about the implications of humanity engineering its own evolution.

In the last few decades, significant advancements in the biology and engineering of stem cells have enabled progress in their clinical application to revascularization therapies. Some strategies involve the mobilization of endogenous stem cell populations, and others employ cell transplantation. However, both techniques have benefited from multidisciplinary efforts to create biomaterials and other biomedical tools that can improve and control the fate of stem cells, and advance our understanding of them. Stem Cells and Revascularization Therapies focuses on the fundamentals and applied studies in stem cell biology, and provides perspectives associated with the development of revascularization strategies. To help readers understand the multidisciplinary issues associated with this topic, this book has been divided into four sections: Section 1: Explores how to define, isolate, and characterize various stem and progenitor cell populations for neovascularization Section 2: Summarizes some especially useful model systems and approaches used to regulate angiogenesis, vasculogenesis, and arteriogenesis, and explores their impact on formation of functional vessels in vivo Section 3: Focuses on stem cell homing to sites of injury and inflammation, as well as strategies to exploit this mobilization phenomenon Section 4: Covers stem cell transplantation topics, including recreating features of endogenous stem cell niches to maintain the multipotency of transplanted cells and combinatorial delivery of cells and molecular factors Intended to inspire new contributions to improve the therapeutic efficacy, Stem Cells and Revascularization Therapies outlines emergent findings and challenges regarding the use of stem cells in revascularization therapies. Overcoming the significant

Summarizing landmark research, Volume 3 of this essential series furnishes information on the availability of germplasm resources that breeders can exploit for producing high-yielding vegetable crop varieties. Written by leading international experts, this volume offers the most comprehensive and up-to-date information on employing genetic resources to increase the yield of those vegetable crops that provide a main source of minerals, vitamins, and antioxidants. In eleven succinct chapters, Genetic Resources, Chromosome Engineering, and Crop Improvement: Vegetable Crops, Volume 3 focuses on potato, tomato, brassicas, okra, capsicum, alliums, cucurbits, lettuce, eggplant, and carrot. An introductory chapter outlines the cytogenetic architecture of vegetable crops, describes the principles and strategies of cytogenetics and breeding, and summarizes landmarks in current research. This sets the stage for the ensuing crop-specific chapters. Each chapter generally provides a comprehensive account of the crop, its origin and taxonomy, wild relatives, exploitation of genetic resources diversity in the primary, secondary, and tertiary gene pools through breeding and cytogenetic manipulation, and genetic enrichment using the tools of molecular genetics and biotechnology. Certain to become the standard reference for improving the yields of these critical vegetable crops, this book is the definitive source of information for plant breeders, gene-bankers, cytogeneticists, taxonomists, molecular biologists, biotechnologists, and graduate students, researchers, agronomists, horticulturists, farmers and consumers in these fields.

Plant Transformation via Agrobacterium Tumefaciens compiles fundamental and specific information and procedures involving in vitro soybean transformation, which forms the basis for the Agrobacterium-mediated genetic manipulation of soybean using plant tissue culture. This method serves as one of the most preferred, reliable and cost-effective mechanism of transgene expression in both leguminous recalcitrant species and non-legume crops. The technology is favoured due to its simplicity, feasibility and high transformation rates that are so far achieved mostly in monocot plants and a few dicot genotypes. This book provides a comprehensive review of plant transformation which remains necessary for many researchers who are still facing protocol-related hurdles. Among some of the major topics covered in Plant Transformation via Agrobacterium Tumefaciens are the history and discovery of Agrobacterium bacterium, longstanding challenges causing transformation inefficiencies, types and conditions of explants, development of transgenic plants for stress resistance, and the role of transgenic plants on animal/human health, including the environment. Plant Transformation via Agrobacterium Tumefaciens helps the reader to understand how soybean, like many other orphan legume crops, faces the risk of overexploitation which may render the currently available varieties redundant and extinct should its narrow gene pool not improve. Plant transformation serves as a key technique in improving the gene pool, while developing varieties that are drought tolerant, have enhanced nutritional value, pest resistant and reduce the destruction by disease causing microorganims. This book is an essential foundation tool that is available for researchers and students to reinforce the application of Agrobacterium-mediated genetic transformation in soybean.

Global demand for wheat, rice, corn, and other essential grains is expected to steadily rise over the next twenty years. Meeting this demand by increasing production through increased land use is not very likely; and while better crop management may make a marginal difference, most agriculture experts agree that this anticipated deficit must be made up through increased crop yields. The first resource of its kind, Physiology and Biotechnology Integration for Plant Breeding assembles current research in crop plant physiology, plant biotechnology, and plant breeding that is aimed toward improving crop plants genetically while supporting a productive agriculture ecosystem. Highly comprehensive, this reference provides access to the most innovative perspectives in crop physiology - with a special emphasis on molecular approaches - aimed at the formulation of those crop cultivars that offer the greatest potential to increase crop yields in stress environments. Surveys the current state of the field, as well as modern options and avenues for plant breeders and biotechnologists interested in augmenting crop yield and stability With the contributions of plant scientists from all corners of the globe who are actively involved in meeting this important challenge, Physiology and Biotechnology Integration for Plant Breeding provides readers with the background information needed to understand this cutting-edge work, as well as detailed information on present and potential applications. While the first half of the book establishes and fully explains the link between crop physiology and molecular biology, the second part explores the application of biotechnology in the effective delivery of the high yield and environmentally stable crop plants needed to avert the very real possibility of worldwide hunger.

Technology for modifying the genotypes and phenotypes of insects and other arthropods has steadily progressed with the development of more precise and powerful methods, most prominently transgenic modification. For many insect pests, there is now almost unlimited ability to modify phenotypes to benefit human health and agriculture. Precise DNA modifications and gene drive have the power to make wild-type populations less harmful in ways that could never have been performed with previous transgenic approaches. This transition from primarily laboratory science to greater application for field use has also necessitated greater development of modeling, ethical considerations and regulatory oversight. The 2nd Edition of Transgenic Insects contains chapters contributed by experts in the field that cover technologies and applications that are now possible. This edition includes increased attention to associated challenges of risk assessment, regulation, and public engagement. Featuring: Up-to-date analysis of molecular techniques, such as gene editing. Consideration of public attitudes and regulatory aspects associated with transgenic insects. Many examples of the wide range of applications of transgenic insects. This book will be very valuable to students and researchers in entomology, molecular biology, genetics, public health and agriculture, and will also appeal to practitioners who are implementing the technology, and to regulators, stakeholders and ethicists.

Summarizing landmark research, Volume 3 of this essential series furnishes information on the availability of germplasm resources that breeders can exploit for producing high-yielding vegetable crop varieties. Written by leading international experts, this volume offers the most comprehensive and up-to-date information on employing genetic resources to increase the yield of those vegetable crops that provide a main source of minerals, vitamins, and antioxidants. In eleven succinct chapters, Genetic Resources, Chromosome Engineering, and Crop Improvement: Vegetable Crops, Volume 3 focuses on potato, tomato, brassicas, okra, capsicum, alliums, cucurbits, lettuce, eggplant, and carrot. An introductory chapter outlines the cytogenetic architecture of vegetable crops, describes the principles and strategies of cytogenetics and breeding, and summarizes landmarks in current research. This sets the stage for the ensuing crop-specific chapters. Each chapter generally provides a comprehensive account of the crop, its origin and taxonomy, wild relatives, exploitation of genetic resources diversity in the primary, secondary, and tertiary gene pools through breeding and cytogenetic manipulation, and genetic enrichment using the tools of molecular genetics and biotechnology. Certain to become the standard reference for improving the yields of these critical vegetable crops, this book is the definitive source of information for plant breeders, gene-bankers, cytogeneticists, taxonomists, molecular biologists, biotechnologists, and graduate students, researchers, agronomists, horticulturists, farmers and consumers in these fields.

Genetic Engineering: A Primer presents the growing field of biotechnology to non-science majors and other general interest readers. The author examines the natural forces that change genetic information and the ways in which scientists have learned to engineer these genetic changes. With a wealth of information flooding the popular press, including news and controversy surrounding cloning, Genetic Engineering is a timely volume that provides background information to the reader intent on understanding this fascinating development.

In China, as elsewhere, the debate over genetically modified organisms has become polarized into anti- and pro-GMO camps. Given the size of China's population and market, much is at stake in conflicts over regulation for domestic as well as international actors. In this book, Cong Cao provides an even-handed analysis that illuminates the tensions that have shaped China's policy toward agricultural biotechnology in a global perspective. Cao presents a comprehensive and systematic analysis of how China's policy toward research and commercialization of genetically modified crops has shifted that explains how China's changing GMO stances reflect its evolving position on the world stage. While China's scientific community has set the agenda, it has encountered resistance rooted in concerns over food safety and consumers' rights as well as issues of intellectual property rights and food sovereignty. Although Chinese leaders at first sought to take advantage of the biotech revolution by promoting GMO crop consumption, Cao demonstrates that policy has since become precautionary, as seen in new laws and regulations grounded in concerns over safety and the deferral of commercialization of GM rice. He presents China's policies in light of changing global attitudes toward GM crops: As shifts in China have closely followed global trends, so has domestic activism. Drawing on government and scientific documents as well as interviews with scientists, officials, policy analysts, activists, and journalists, GMO China is an important book for China studies, science and technology studies, policy analysts, and professionals interested in the Chinese biotechnology market.