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Electroporation Protocols for Microorganisms (Paperback, 1995 ed.)
Loot Price: R2,374
Discovery Miles 23 740
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Electroporation Protocols for Microorganisms (Paperback, 1995 ed.)
Series: Methods in Molecular Biology, 47
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Electroporation is one of the most widespread techniques used in
modem molecular genetics. It is most commonly used to introduce DNA
into cells for investigations of gene structure and function, and
in this regard, electroporation is both highly versatile, being
effective with nearly all species and cell types, and highly
efficient. For many cell types, electroporation is either the most
efficient or the only means known to effect gene transfer. However,
exposure of cells to brief, hi- intensity electric fields has found
broad application in other aspects of biological research, and is
now routinely used to introduce other types of biological and
analytic molecules into cells, to induce cell-cell fusion, and to
transfer DNA directly between different species. The first seven
chapters of Electroporation Protocols for Micro organisms describe
the underlying theory of electroporation, the com mercially
available instrumentation, and a number of specialized
electroporation applications, such as cDNA library construction and
interspecies DNA electrotransfer. Each of the remaining chapters
pre sents a well developed method for electrotransformation of a
particular bacterial, fungal, or protist species. These chapters
also serve to intro duce those new to the field the important
research questions that are currently being addressed with
particular organisms, highlighting both the major advantages and
limitations of each species as a model organ ism, and explaining
the roles that electroporation has played in the development of the
molecular genetic systems currently in use."
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