Having a robust drought monitoring system for Ethiopia is crucial to mitigate the adverse impacts of droughts. Yet, such monitoring system still lacks in Ethiopia, and in the Upper Blue Nile (UBN) basin in particular. Several drought indices exist to monitor drought, however, these indices are unable, individually, to provide concise information on the occurrence of meteorological, agricultural and hydrological droughts. A combined drought index (CDI) using several meteorological, agricultural and hydrological drought indices can indicate the occurrence of all drought types, and can provide information that facilitates the drought management decision-making process. This thesis proposes an impact-based combined drought index (CDI) and a regression prediction model of crop yield anomalies for the UBN basin. The impact-based CDI is defined as a drought index that optimally combines the information embedded in other drought indices for monitoring a certain impact of drought, i.e. crop yield for the UBN. The developed CDI and the regression model have shown to be effective in indicating historic drought events in UBN basin. The impact-based CDI could potentially be used in the future development of drought monitoring in the UBN basin and support decision making in order to mitigate adverse drought impacts.

The aim of this Ph.D. was to develop a technology for the remediation of seleniferous soils/sediments and to explore microbial reduction of selenium oxyanions under different respiration conditions and bioreactor configurations. Seleniferous soil collected from the wheat-grown agricultural land in Punjab (India) was characterized and its soil washing was optimized by varying parameters, where addition of oxidizing agents showed a maximum selenium removal efficiency. Aquatic plants, Lemna minor and Egeria densa were used to study phytoremediation of the selenium-rich soil leachate containing oxidizing agents. Additionally, migration of the soluble selenium fraction from the upper to the lower layers and its subsequent reduction and accumulation in the lower layers of the soil column was observed during soil flushing. Furthermore, the soil leachate containing selenium oxyanions obtained from soil washing was treated in a UASB reactor by varying the organic feed. Ex situ bioremediation of selenium oxyanions was studied under variable conditions. An aerobic bacterium (Delftia lacustris) capable of transforming selenate and selenite to elemental selenium was isolated and characterized. Anaerobic bioreduction of selenate coupled to methane oxidation was investigated in serum bottles and a biotrickling filter using marine sediment as inoculum. Finally, the effect of contamination of other chalcogen oxyanions (tellurium) on selenium bioreduction was studied in a continuous system (UASB reactor).

The Congress "Arsenic in the Environment" offers an international, multi- and interdisciplinary discussion platform for research aimed towards a holistic solution to the problem posed by the environmental toxin arsenic, with considerable societal impact. The congress has focused on cutting edge and breakthrough research in physical, chemical, toxicological, medical, agricultural and other specific issues on arsenic across a broader environmental realm. The Congress "Arsenic in the Environment" was first organized in Mexico City (As2006) followed by As2008 in Valencia, Spain, As2010 in Tainan, Taiwan and As2012 in Cairns, Australia. The 5th International Congress As2014 was held May 11-16, 2014 in Buenos Aires, Argentina and entitled One Century of the Discovery of Arsenicosis in Latin America (1914-2014). The session topics comprised: Theme 1: Arsenic in environmental matrices (air, water and soil) Theme 2: Arsenic in food Theme 3: Arsenic and health Theme 4: Removal technologies Theme 5: Mitigation management and policy Hosting this Congress in Argentina was especially relevant because 2014, marks 100 years since the discovery of the disease Hidroarsenicismo Cronico Regional Endemico (HACRE) or arsenicosis by Dr. Goyenechea and Dr. Ayerza in the city of Bell Ville, Province of Cordoba, Argentina. Dr. Ayerza was the first person to relate skin disorders to the consumption of groundwater with high concentrations of arsenic. It is estimated that more than 14 million people in Latin America are at risk, of whom nearly 4 million are exposed to drinking water with high arsenic concentration in Argentina, and further in Chile, El Salvador, Mexico, Nicaragua and Peru. A vast area of the Chaco-Pampean Plain in Argentina, mostly in the semi-arid regions, is affected not only by arsenic exposure from drinking water but also through other exposure pathways, e.g. through food and other dietary intake. The Congress has gathered professionals involved in different segments of interdisciplinary research in an open forum, and strengthens relations between academia, industry, research laboratories, government agencies and the private sector to share an optimal atmosphere for exchange of knowledge, discoveries and discussions about the problem of arsenic in the environment.

Water resources in tourist islands have been severely threatened, especially in the Galapagos Islands, where the increased local population has generated attractive income from the tourist services. In addition, the data regarding water supply and demand are scarce. This study investigates water supply and demand in Santa Cruz, the most populated island of Galapagos. The research encompasses a thorough assessment of the water supply crisis, as well as the quantification of water demand from different categories (domestic, tourist, restaurants and laundries) through surveys, in the absence of water metering. Also, specific water demand was assessed by installing 18 water meters. The results yield a wide range of water consumption, questioning the current assumption of water scarcity. Furthermore, a prognosis of water supply and demand was carried out, and also several intervention strategies were proposed such as rainwater harvesting, greywater recycling, leakage reduction, water meter installation, water demand reduction, as well as seawater desalination to cope with the future population growth. Due to the fragility of the ecosystem, these strategies were assessed through a Multi-Criteria Decision Analysis, considering environmental, technical, economic and social aspects, as well as relevant stakeholders' perspectives. finally, the water supply network of Puerto Ayora was evaluated in order to understand the need of the current intermittent supply regime. A methodology was developed to estimate the overflow of the domestic roof tanks (a common incidence amongst local population). The results question the practicality of individual household storage. The final results show that the current situation in terms of the lack of water quantity may not be real, as it has been thought for the last decades. The water issues refer more importantly to the water quality, as well as to the lack of proper water management practices.

A considerable amount of scientific evidence has been collected leading to the conclusion that urban wastewater components should be designed as one integrated system, in order to protect the receiving waters cost-effectively. Moreover, there is a need to optimize the design and operation of the sewerage network and wastewater treatment plant (WwTP) considering the dynamic interactions between them and the receiving waters. This book introduces a method called Model Based Design and Control (MoDeCo) for the optimum design and control of urban wastewater components. The book presents a detailed description of the integration of modelling tools for the sewer, the wastewater treatment plants and the rivers. The complex modelling structure used for the integrated model challenge previous applications of integrated modelling approaches presented in scientific literature. The combination of modelling tools and multi-objective evolutionary algorithms demonstrated in this book represent an excellent tool for designers and managers of urban wastewater infrastructure. This book also presents two alternatives to solve the computing demand of the optimization of integrated systems in practical applications: the use of surrogate modelling tools and the use of cloud computer infrastructure for parallel computing.

Urban flood risks and their impacts are expected to increase as urban development in flood prone areas continues and rain intensity increases as a result of climate change while aging drainage infrastructures limit the drainage capacity in existing urban areas. Flood mitigation strategies are required as part of sound urban flood management plans to assess flood risk and identify technically feasible and cost-effective options to reduce the risk. Central to the development of flood mitigation strategies is the efficient prediction of flood propagation characteristics in urban areas. Detailed predictions of flood flows in urban areas require the use of high resolution topographic data. However, due to computational demand topographic data is often generalised to a more manageable resolution and floodplain models are built at much coarser resolutions. The research presented in this thesis addresses the problem of capturing small-scale features in coarse resolution urban flood models with the aim of improving flood forecasts in geometrically complex urban environments. The approach adopted in the research used a two-dimensional surface flow modelling system that can extract and incorporate useful information available in high resolution topographic data into coarse grid models. The thesis also presents modelling of the complex interaction between surcharged sewer and flows associated with urban flooding and incorporation of infiltration process in surface flow modelling tools. Case study results showed that the incorporating volume-depth and flow-area-depth relationships extracted from high resolution topographic data significantly improved the results of coarse grid urban flood models while taking the advantage of reduced computational time to ensure efficient prediction of urban flood characteristics.

Urban informal settlements or slums are growing rapidly in cities in sub-Saharan Africa. Most often, a sewer system is not present and the commonly-used low-cost onsite wastewater handling practices, typically pit latrines, are frequently unplanned, uncontrolled and inefficient. Consequently, most households dispose of their untreated or partially treated wastewater on-site, generating high loads of nutrients to groundwater and streams draining these areas. However, the fate of nutrients in urban slums is generally unknown. In excess, these nutrients can cause eutrophication in downstream water bodies. This book provides an understanding of the hydro-geochemical processes affecting the generation, fate and transport of nutrients (nitrogen and phosphorus) in a typical urban slum area in Kampala, Uganda. The approach used combined experimental and modeling techniques, using a large set of hydrochemical and geochemical data collected from shallow groundwater, drainage channels and precipitation. The results show that both nitrogen-containing acid precipitation and domestic wastewater from slum areas are important sources of nutrients in urban slum catchments. For nutrients leaching to groundwater, pit latrines retained over 80% of the nutrient mass input while the underlying alluvial sandy aquifer was also an effective sink of nutrients where nitrogen was removed by denitrification and anaerobic oxidation and phosphorus by adsorption to calcite. In surface water, nutrient attenuation processes are limited. This study argues that groundwater may not be important as regards to eutrophication implying that management interventions in slum areas should primarily focus on nutrients released into drainage channels. This research is of broad interest as urbanization is an ongoing trend and many developing countries lack proper sanitation systems.

The rate of global increase in water abstraction for irrigation has been declining since the 1970's due to declining potentials for large and medium-scale irrigation developments, and is expected to further decline in the next decades. As such the significant proportion of the expected increase in production would have to be supplied from existing irrigated and /or cultivated lands. This in turn could be achieved by enhancing land and water productivity through improved performance and optimal operation and maintenance. With less than 15% of over 5 million ha irrigation potential harnessed, irrigation devolvement in Ethiopia remained low. Over 70% of the developed irrigation in the country belongs to small-scale irrigation serving smallholder farmers. While accelerated development of new irrigation, particularly of large and medium-scale schemes is relevant in Ethiopia, ensuring the performance and sustainability of existing schemes is also equally important. The existing irrigation schemes in Ethiopia are generally characterized by an overall performance and technical sustainability levels of below expectation. This thesis evaluates the performance of two large-scale (Wonji-Shoa and Metahara) and two community-managed (Golgota and Wedecha) irrigation schemes located in the Awash River Basin of Ethiopia. The study focussed on hydraulic/water delivery performance in the large-scale schemes, and on comparative and internal irrigation service (utility) evaluation in the community-managed schemes. Water delivery performance was evaluated using routine data and hydrodynamic modelling. Farmers' utility was evaluated using qualitative responses of water users. Major performance challenges in each category of schemes were addressed and operational/water management options for improvement were identified.

Africa has been severely affected by droughts in the past, contributing to food insecure conditions in several African countries. In view of the (even more) severe drought conditions and water shortage that may be expected in sub-Saharan Africa in the coming years, efforts should focus on improving drought management by ameliorating resilience and preparedness to drought. This study contributes to the development of a modelling framework for hydrological drought forecasting in sub-Saharan Africa as a step towards an effective early warning system. The proposed hydrological drought forecasting system makes use of a hydrological model that was found to be suitable for drought forecasting in Africa and could represent the most severe past droughts in the Limpopo Basin. The modelling results showed that there is an added value in computing indicators based on the hydrological model for the identification of droughts and their severity. The proposed seasonal forecasting system for the Limpopo Basin was found to be skilful in predicting hydrological droughts during the summer rainy season. The findings showed that the persistence of the initial hydrological conditions contribute to the predictability up to 2 to 4 months, while for longer lead times the predictability of the system is dominated by the meteorological forcing. An effective drought forecasting and warning system will hopefully contribute to important aspects in the region such as water security, food security, hazard management, and risk reduction.

This work investigated two different approaches to optimize biological sulphate reduction in order to develop a process control strategy to optimize the input of an electron donor and to study how to increase the feasibility of using a cheap carbon source. Feast/famine regimes, applied to design the control strategy, were shown to induce the accumulation of storage compounds in the sulphate reducing biomass. This study showed that delays in the response time and a high control gain can be considered as the most critical factors affecting a sulphide control strategy in bioreactors. The delays are caused by the induction of different metabolic pathways in the anaerobic sludge, including the accumulation of storage products. On this basis, a mathematical model was developed and validated. This can be used to develop optimal control strategies. In order to understand the microbial pathways in the anaerobic oxidation of methane coupled to sulphate reduction (AOM-SR), diverse potential electron donors and acceptors were added to in vitro incubations of an AOM-SR enrichment at high pressure. Acetate was formed in the control group, probably resulting from the reduction of CO2. These results support the hypothesis that acetate may serve as an intermediate in the AOM-SR process.

The aim of this book is to contribute to understanding risk knowledge and to forecasting components of early flood warning, particularly in the environment of tropical high mountains in developing cities. This research covers a challenge, taking into account the persistent lack of data, limited resources and often complex climatic, hydrologic and hydraulic conditions. In this research, a regional method is proposed for assessing flash flood susceptibility and for identifying debris flow predisposition at the watershed scale. An indication of hazard is obtained from the flash flood susceptibility analysis and continually, the vulnerability and an indication of flood risk at watershed scale was obtained. Based on risk analyses, the research follows the modelling steps for flood forecasting development. Input precipitation is addressed in the environment of complex topography commonly found in mountainous tropical areas. A distributed model, a semi-distributed model and a lumped model were all used to simulate the discharges of a tropical high mountain basin with a paramo upper basin. Performance analysis and diagnostics were carried out in order to identify the most appropriate model for the study area for flood early warning. Finally, the Weather Research and Forecasting (WRF) model was used to explore the added value of numerical weather models for flood early warning in a paramo area.

For integrated water resources management both blue and green water resources in a river basin and their spatial and temporal distribution have to be considered. This is because green and blue water uses are interdependent. In sub-Saharan Africa, the upper landscapes are often dominated by rainfed and supplementary irrigated agriculture that rely on green water resources. Downstream, most blue water uses are confined to the river channels, mainly for hydropower and the environment. Over time and due to population growth and increased demands for food and energy, water use of both green and blue water has increased. This book provides a quantitative assessment of green-blue water use and their interactions. The book makes a novel contribution by developing a hydrological model that can quantify not only green but also blue water use by many smallholder farmers scattered throughout the landscape. The book provides an innovative framework for mapping ecological productivity where gross returns from water consumed in agricultural and natural vegetation are quantified. The book provides a multi-objective optimization analysis involving green and blue water users, including the environment. The book also assesses the uncertainty levels of using remote sensing data in water resource management at river basin scale.

The enhanced biological removal of phosphorus (EBPR) is a popular process due to high removal efficiency, low operational costs, and the possibility of phosphorus recovery. Nevertheless, the stability of the EBPR depends on different factors such as: temperature, pH, and the presence of toxic compounds. While extensive studies have researched the effects of temperature and pH on EBPR systems, little is known about the effects of different toxic compounds on EBPR. For example, sulphide has shown to inhibit different microbial activities in the WWTP, but the knowledge about its effects on EBPR is limited. Whereas the sulphide generated in the sewage can cause a shock effect on EBPR, the continuously exposure to sulphide potentially generated in WWTP can cause the acclimatization and adaptation of the biomass. This research suggests that sulphate reducing bacteria can proliferate in WWTP, as they are reversibly inhibited by the recirculation of sludge through anaerobic-anoxic-oxic conditions. The research enhances the understanding of the effect of sulphide on the anaerobic-oxic metabolism of PAO. It suggests that the filamentous bacteria Thiothrix caldifontis could play an important role in the biological removal of phosphorus. It questions the ability of PAO to generate energy from nitrate respiration and its use for the anoxic phosphorus uptake. Thus, the results obtained in this research can be used to understand the stability of the EBPR process under anaerobic-anoxic-oxic conditions, especially when exposed to the presence of sulphide.

Quantitative research with respect to the combination of engineering and socialcultural- religious aspects based on the Tri Hita Karana philosophy in Subak irrigation schemes is original in the field of land and water development. A scenario analysis needs a good and careful system approach. Based on a Generic Algorithm the RIBASIM model was applied using the dependable 80% of discharge and shifting the start of land preparation. The results provide evidence that the cropping pattern of the fifth scenario results in an overall optimal agriculture production of the Subak schemes. The recoverable flow considered in the river basin scheme model plays an important role in the optimisation. Nevertheless, if a normal hydro-climate occurs, the other scenarios, especially the first scenario, can be applied as well. When the indigenous knowledge of farmers is compromised with present day knowledge of agricultural and technological developments, capability of these farmers increases, thus reflects the applicability of the Tri Hita Karana philosophy on harmony among people and harmony among people and nature.

The book presents documentary evidence of the insufficiency of rehabilitation works to close the gap between the irrigation service and actual area irrigated of publicly funded national irrigation systems in the Philippines. It outlines a methodology for formulating a modernisation plan for national irrigation systems with focus on the mostly ungauged, medium to small canal irrigation systems. The proposed methodology adaptively modified some known modernisation concepts and techniques and integrated them in a more holistic framework in the context of changing weather patterns and river flow regimes. It includes in-depth review of rehabilitation works; system diagnosis; revalidation of design assumptions on percolation and water supply; characterisation of system management, irrigation service and demand; and drawing up of options and a vision for the modernised irrigation systems. Central to the proposed modernisation strategy is the logical coherence among the design of physical structures, system operation and water supply so that improvements of irrigation service are possible. The book discusses the development of the proposed methodology and demonstrates its utility in two case study irrigation systems.

The unprecedented growth of cities has a significant impact on future flood risk that might exceed the impacts of climate change in many metropolitan areas across the world. Although the effects of urbanisation on flood risk are well understood, assessments that include spatially explicit future growth projections are limited. This comparative study provides insight in the long term development of future riverine and pluvial flood risk for 18 fast growing megacities. The outcomes provide not only a baseline absent in current practise, but also a strategic outlook that might better establish the role of urban planning in limiting future flood risk.

The role of small hydropower is becoming increasingly important on a global level. Increasing energy demand and environmental awareness has further triggered research and development into sustainable low-cost technologies. In developing countries, particularly in rural areas, the possibility of local power generation could considerably improve living conditions. With this in mind, the development of a next generation low-head hydropower machines was subject of investigation in the EU-project HYLOW. Being part of the research lines of that project, this thesis presents a numerical modelling approach to improve the design of machines like water wheels for increased hydraulic efficiency. Nowadays, Computational Fluid Dynamics (CFD) enables numerical models to be quite accurate and incorporate physical complexities like free surfaces and rotating machines. The results of the CFD simulations carried out in this research show that a change in blade geometry can result in higher torque levels, thereby increasing performance. Numerical simulations also enabled to determine the optimal wheel-width to channel-width ratio and further improve performance by modifying the channel bed conditions upstream and downstream of the water wheel. With a power rating in the low kilowatt range, low-head hydropower machines like optimised water wheels seem to have a clear potential for small-scale energy generation, thereby contributing to achieving the Sustainable Development Goals by providing local energy solutions.

International cooperation for reaching development goals has expanded gradually since the 1950s. The effectiveness of the Overseas Development Aid (ODA) has become a topic of great public interest. A growing body of experience exists to demonstrate that finance alone is not sufficient for development, and capacity and knowledge are increasingly seen as the constraints to proper decision making, absorption of funds, and effective results on the ground. This book presents the investigation into the role of Knowledge and Capacity Development (KCD) in public water resources management in Indonesia and the Netherlands. The two cases indicate that the institutional environment is defining for the type of knowledge and capacity development that is prevailing in that period and it also determines the formal organisational structure and the KCD mechanisms in use. The cases further show the importance yet the low valuation of tacit knowledge, while the Indonesian case also suggests that tacit knowledge is among the most important assets gained from international post-graduate education. The conceptual model introduced in this book allows to measure knowledge and capacity at three nested levels: the institutional, organisational and individual level. It provides insight in the numerous contextual factors that influence knowledge and capacity and KCD mechanisms.

Mountains are water towers of our world, but their role in global water resources may be altered due to changing climate. This book provides an integrated assessment of the spatial and temporal variability of both recent and future climate change impacts in the Yellow River source region (YRSR) with specific focus on extremes. The book is structured across four different topics from detecting contemporary hydro-climatic changes, comparing three different statistical downscaling methods, assessing elevation dependency of expected changes in temperature, and projecting future climate-induced hydrologic changes in the YRSR. The detection of historical hydro-climatic changes in recent decades indicates that climate change may already be happening and may pose a serious threat to water availability in this region. However, an ensemble of climate change projections for the periods 2046-2065 and 2081-2100 based on two GCMs and three emission scenarios demonstrates that the future water availability of this region would increase due to climate change. This discrepancy suggests that contemporary hydro-climatic experience based on past records alone may not always provide a reliable guide to the future. This study makes an important contribution toward an improved understanding of climate change impacts in the YRSR. The knowledge generated has major implications for water resources management in the Yellow River and will be instructive for climate change impacts studies in other mountain areas.

After World War II, international development became the world leading development model, but its effectiveness is much debated. Nowadays, it is acknowledged that international development can effectively support development through knowledge and capacity development (KCD). Nonetheless, understanding what capacity really consists of in operational terms and what its development entails remains a challenge. This book investigates KCD processes in water utilities in Sub-Saharan Africa. The three cases analysed reveal that the learning impact of KCD on utilities depends on whether they are able to close their learning cycle, i.e., to ensure that improved capacity is also translated into mainstream behaviour. This finding challenges conventional wisdom for which KCD translates "automatically" into improved performance. Hence the need to focus KCD evaluation on both capacity improvement and capacity application. The proposed learning-based framework for KCD identifies two distinct but interrelated stages in KCD, namely knowledge transfer and knowledge absorption. Knowledge absorption usually takes time due to slow organisational processes that govern it. However, in practice it is often taken for granted. The framework also identifies the key factors that shape learning processes in water utilities. The book argues that water utilities in Sub-Saharan Africa can reinvent themselves by embracing change management approaches and striving to become learning organisations.

Providing an introduction to the crucially important topic of groundwater, this text covers all major fields of hydrogeology and includes outlines of the occurrence of groundwater in various rock types, the movement and storage of groundwater, the formulation of groundwater balances, the development of groundwater chemistry, as well as the practical application of hydrogeology for groundwater development. Following a unique systems approach to describe and connect its various elements, the text also explores a large selection of examples of groundwater cases from various parts of the world. In addition, theoretical sections and examples are illustrated with a number of drawings, photos and computer printouts. Suitable for education in hydrogeology at postgraduate and graduate level, the text is also a useful reference tool for professionals and decision-makers involved in water or water-related activities. In the revised paperback edition more attention is paid to the processes in the unsaturated zone, especially those relating to groundwater recharge. Also, the investigation methods are highlighted in the sections where the related theory is dealt with, and they are not presented in the last chapter on groundwater management. The References and Bibliography section is also extended, some figures are improved, and the inevitable 'typing errors' are corrected as well. In the third edition, a more formal basis for the hydro-chemical processes described in the chapter on groundwater chemistry has been added. Mass balances and the principles of dispersion and retardation are introduced. Additional illustrations are provided, also explaining the processes occurring along streamlines. Consult: http://introductiontohydrogeology.nl/ for additional information on the book, the author and available software.

Since the 1980s a major change took place in public policies for water resources management. The role of governments shifted under this reform process from an emphasis on investment in the development, operation and maintenance of water infrastructure to a focus on managing water resources systems by stipulating general frameworks and defining key principles for water allocation. This interdisciplinary research examines how this water reform process unfolds within four African waterscapes that are historically constituted by natural and social processes. The study analyzes the interplay between public policies designed and implemented by government agencies and the institutions that govern access to and control over water resources among groups of agricultural water users. The findings of this research show that the water reform policies have led to similar outcomes in dissimilar contexts and that water policy only to a limited extent leads to progressive institutional change concerning agricultural water use, especially in this neoliberal era. Moreover, this research shows that excluding targeted investments in the development of hydraulic infrastructure for historically disadvantaged groups has narrowed the options of the governments to redress the colonial legacy and the capacity of small-scale farmers to move their livelihood beyond subsistence.

Sediment pollution and accumulation in harbours are major environmental issues and studies that advance their solutions are essential for harbour sustainability. This book provides the first comprehensive assessment of chemical pollution in sediments and sediment accumulation rates in the tropical Tema Harbour (Ghana). This book contributes to improving our ability to use an integrated approach involving sediment chemistry and bioassays in one comprehensive assessment of the contamination state of a tropical coastal environment. Whole-sediment toxicity bioassays using the amphipod Corophium volutator and the polychaete Hediste diversicolor as bioindicators were combined with data on concentrations of total metal and metal binding forms, radionuclides, organochlorine pesticides and polycyclic aromatic hydrocarbons in bottom sediments as well as total metal concentrations in settling silt-clay particles collected by sediment traps to characterise the hazard, risk and impact of sediments from the tropical coastal Tema Harbour.

This research investigated new approaches to control anaerobic methane oxidation coupled to sulfate reduction (AOM-SR) and enrich anaerobic methanotrophs (ANME) and sulfate reducing bacteria (SRB) with the purpose of designing a suitable bioreactor for AOM-SR at ambient pressure and temperature. The current knowledge about AOM and the microorganisms involved in AOM are discussed. The effect of different substrates and pressures was investigated on the ANME and SRB community adapted to the shallow marine Lake Grevelingen, the Netherlands. Further, microorganisms from the Alpha Mound (Spain) deep sediment were enriched with methane gas as substrate in biotrickling filters (BTF) at ambient conditions for 147-230 days of operation. The effect of alternative sulfur compounds (sulfate, thiosulfate and elemental sulfur) were studied and the microbial community was characterized. The highest AOM and sulfate reduction rates were obtained in the BTF fed with thiosulfate as the electron acceptor (~0.4 mmol l-1 day-1), but the highest number of ANME was visualized in the sulfate fed BTF (ANME-2 43% of the total visualized archaea). A BTF was proposed as a suitable bioreactor for the enrichment of ANME and SRB at ambient pressure and temperature which could be potentially used for future biotechnological applications.

Anaerobic oxidation of methane (AOM) coupled to sulfate reduction (AOM-SR) is a biological process mediated by anaerobic methanotrophs (ANME) and sulfate reducing bacteria. It has scientifi c and societal relevance in regulating the global carbon cycle and biotechnological application for treating sulfate-rich wastewater. This research aimed to enhance the recent knowledge on ANME distribution and its enrichment in different bioreactor confi gurations, i.e. membrane bioreactor (MBR), biotrickling fi lter (BTF) and high pressure bioreactor (HPB). Marine sediment from Ginsburg mud volcano, Gulf of Cadiz was used as inoculum in the BTF and MBR. The BTF operation showed the enrichment of ANME in the biofi lm, especially ANME-1 (40%) and ANME-2 (10%). Whereas, the dominancy of ANME-2 and Desulfosarcina aggregates was observed in the MBR. Moreover, HPB study was performed by using highly enriched ANME-2 community from Captain Arutyunov mud volcano. During the study of HPB at different temperature and pressure conditions, the incubation at 10 MPa pressure and 15 C was observed to be the most suitable condition for the studied AOM-SR community. Furthermore, AOM-SR activity in the coastal sediments from marine Lake Grevelingen (the Netherlands) was explored and the microbial community was characterised which was dominated by ANME-3 among known ANME types.