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Production of Membrane Proteins - Strategies for Expression and Isolation (Hardcover)
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Production of Membrane Proteins - Strategies for Expression and Isolation (Hardcover)
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Designed as a research-level guide to current strategies and
methods of membrane protein production on the small to intermediate
scale, this practice-oriented book provides detailed, step-by-step
laboratory protocols as well as an explanation of the principles
behind each method, together with a discussion of its relative
advantages and disadvantages.
Following an introductory section on current challenges in membrane
protein production, the book goes on to look at expression systems,
emerging methods and approaches, and protein specific
considerations.
Case studies illustrate how to select or sample the optimal
production system for any desired membrane protein, saving both
time and money on the laboratory as well as the technical
production scale.
Unique in its coverage of "difficult" proteins with large
membrane-embedded domains, proteins from extremophiles, peripheral
membrane proteins, and protein fragments.
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