Our approach to the way we look at genes or analyze DNA completely
revolutionized after Dr. Kary Mullis envisioned a means of copying
DNA in a chain reaction in 1983. Subsequently, he won the Nobel
Prize for inventing this powerful polymerase chain reaction (PCR)
technology which has now become a ubiquitous tool in every life
science laboratory, be it for basic or applied research, for
diagnostics.PCR is one of the most important techniques used to
study DNA and RNA obtained from a variety of tissue sources and
thus has become a "Cornerstone of Genome Sequencing Projects."
Today the extent to which immune-pathologic and molecular
pathologic techniques are employed varies greatly, but it is
conceivable that in the next decade, many of today's most
technically advanced methods of molecular analysis will become
standard practice. The aim here is to provide an overall
familiarity with PCR, details of some typical PCR experiments, and
a focus on issue useful to the field of genomic analysis.
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