There has been a great upsurge in interest in light microscopy in
recent years due to the advent of a number of significant advances
in microscopy, one of the most important of which is confocal
microscopy. Confocal microscopy has now become an important
research tool, with a large number of new fluorescent dyes becoming
available in the past few years, for probing your pet structure or
molecule within fixed or living cell or tissue sampies. Many of the
people interested in using confocal microscopy to further their
research do not have a background in microscopy or even cell
biology and so not only do they find considerable difficulty in
obtaining satisfactory results with a confocal microscope, but they
may be mislead by how data is being presented. This book is
intended to teach you the basic concepts ofmicroscopy,
fluorescence, digital imaging and the principles of confocal
microscopy so that you may take full advantage ofthe excellent
confocal microscopes now available. This book is also an excellent
reference source for information related to confocal microscopy for
both beginners and the more advanced users. For example, do you
need to know the optimal pinhole size for a 63x 1. 4 NA lens? Do
you need to know the fluorescence emission spectrum of Alexa 568?
Access to the wealth of practical information in this book is made
easier by using both the detailed index and the extensive glossary.
General
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