Spatio-temporal expression of insecticidal gene (Cry1Ac& Cry2A)
in pre existing lines of transgenic cotton was studied. Seasonal
decline in expression of genes differed significantly among
different cotton lines tested in the field conditions. The leaves
of the Bt cotton plants were found to have the highest levels of
toxin expression followed by squares, bolls, anthers and petals.
Expression of the genes decreased consistently with the age of
plants. Toxin expression in fruiting parts was not enough to confer
full resistance against bollworms. The reduction in efficacy of
transgenic cotton plants late in the season was attributed to
reduction in promoter activity. For this purpose, Rubisco small
subunit promoter was isolated from Gossypium arboreum that was
further cloned upstream of Cry1Ac in expression vector pCAMBIA
1301. A local cotton cultivar was transformed with Cry1Ac driven by
rbcS promoter. The same cotton cultivar was also transformed with
Cry1Ac gene driven by 35SCaMV promoter to compare the expression
pattern of insecticidal gene under two different promoters. The
results showed that rbcS is an efficient promoter to drive Bt gene
expression in cotton.
General
Imprint: |
Lap Lambert Academic Publishing
|
Country of origin: |
Germany |
Release date: |
December 2011 |
First published: |
December 2011 |
Authors: |
Allah Bakhsh
• Tayyab Husnain
|
Dimensions: |
229 x 152 x 10mm (L x W x T) |
Format: |
Paperback - Trade
|
Pages: |
176 |
ISBN-13: |
978-3-8443-8920-3 |
Categories: |
Books
|
LSN: |
3-8443-8920-2 |
Barcode: |
9783844389203 |
Is the information for this product incomplete, wrong or inappropriate?
Let us know about it.
Does this product have an incorrect or missing image?
Send us a new image.
Is this product missing categories?
Add more categories.
Review This Product
No reviews yet - be the first to create one!