In ELISA formats there are several parameters that are common and
critical to assay performance and should be considered during the
optimization process, including antibodies concentration, antigen
concentration, incubation time of blocking, substrate solution and
washing solution. Though immunoassays offer a specific, sensitive
and rapid method to detect and quantify even trace amounts of
allergens in foodstuffs, certain processes can denature, alter or
destroy proteins so that they are no longer detectible by assay
using antibodies. When using monoclonal antibodies, there is a
great chance of obtaining false negatives in the detection.
Polyclonal antibodies are recommended for ELISA techniques as the
risk of obtaining false negatives is reduced as compared to
monoclonal antibodies which are quite specific.
General
Imprint: |
Lap Lambert Academic Publishing
|
Country of origin: |
Germany |
Release date: |
March 2012 |
First published: |
March 2012 |
Authors: |
Amjad Iqbal
|
Dimensions: |
229 x 152 x 5mm (L x W x T) |
Format: |
Paperback - Trade
|
Pages: |
80 |
ISBN-13: |
978-3-8484-3663-7 |
Categories: |
Books
|
LSN: |
3-8484-3663-9 |
Barcode: |
9783848436637 |
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