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Nonribosomal Peptide and Polyketide Biosynthesis - Methods and Protocols (Hardcover, 1st ed. 2016)
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Nonribosomal Peptide and Polyketide Biosynthesis - Methods and Protocols (Hardcover, 1st ed. 2016)
Series: Methods in Molecular Biology, 1401
Expected to ship within 10 - 15 working days
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This volume presents an overview of nonribosomal peptide synthetase
(NRPS) and polyketide synthase (PKS) structure and function. It
then continues with methods for the analysis of these pathways
including conventional enzymological assays, contemporary mass
spectrometric analysis techniques, specialized molecular biological
approaches applicable to NRPSs and PKSs, and small molecule
analysis tools tailored to this very special class of natural
products, and concludes by examining bioinformatics tools for the
analysis of these enzymes, pathways, and molecules. Written for the
highly successful Methods in Molecular Biology series, chapters
include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Authoritative and practical, Nonribosomal
Peptide and Polyketide Biosynthesis: Methods and Protocols serves
as a valuable reference for those experienced in studying NRPS and
PKS enzymes, pathways, and natural products as well as a gateway
for those just entering the field.
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