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siRNA Design - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2013) Loot Price: R4,439
Discovery Miles 44 390
siRNA Design - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2013): Debra J. Taxman

siRNA Design - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2013)

Debra J. Taxman

Series: Methods in Molecular Biology, 942

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Loot Price R4,439 Discovery Miles 44 390 | Repayment Terms: R416 pm x 12*

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The discovery of RNA interference (RNAi) as a methodology for gene silencing has revolutionized biological research, providing an invaluable avenue for therapeutics, and small interfering RNA (siRNA) is the most common strategy utilized for enacting RNAi. siRNA Design: Methods and Protocols offers expertly crafted guidelines and protocols for the selection of siRNA targeting sequences, for the strategic incorporation of chemical modifications, and for advantageous structural modifications to the classic siRNA design. Protocols are provided for using endogenous cellular machinery to produce siRNA from optimized precursor short hairpin RNA (shRNA) and artificial microRNA (amiRNA) molecules. Strategies are also described for specific applications such as immunostimulatory siRNA that may provide therapeutic benefit against viral infections in mammals, the simultaneous targeting of multiple siRNAs, and siRNA-mediated crop virus resistance. The design of RNAi for gene silencing in embryonic, invertebrate, and plant systems requires a variety of unique approaches, several of which are described towards the end of this volume. Written for the highly successful Methods in Molecular Biology (TM) series, this work contains the kind of detailed description and implementation advice that guarantees successful results. Authoritative and easy to use, siRNA Design: Methods and Protocols will provide researchers, educators, clinicians, and biotech specialists with a broad understanding of the issues in RNAi and how they can be overcome strategically through design.

General

Imprint: HumanaPress
Country of origin: United States
Series: Methods in Molecular Biology, 942
Release date: August 2016
First published: 2013
Editors: Debra J. Taxman
Dimensions: 254 x 178 x 21mm (L x W x T)
Format: Paperback
Pages: 392
Edition: Softcover reprint of the original 1st ed. 2013
ISBN-13: 978-1-4939-5978-5
Categories: Books > Medicine > Pre-clinical medicine: basic sciences > Medical genetics
Books > Professional & Technical > Biochemical engineering > Biotechnology > General
LSN: 1-4939-5978-6
Barcode: 9781493959785

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