It is important new text book, sharply focous on begomoviruses. It
is first report from Pakistan. The sequences obtained in this study
were compared with reported sequence in NCBI database using BLAST
analysis and preliminary results showed that clone FQ-1 is a strain
of MeYVMV and FQ-2, FQ-3 and FQ-4 are isolates of AEV. Sequence
FQ-1 were downloaded and used for sequence comparison in MegAlign
by Clustal V method. MegAlign results showed that clone FQ-1 has
between 89.9 and 91.9% identity to MeYVMV. It encodes six ORF
typical of Old World begomoviruses. The complete sequence of FQ-1
is submitted to NCBI databank under accession no. HE578897.
Phylogenetic analysis showed that sequence of obtained in this
study has a distinct position in the phylogenetic tree and it
appears to be among the ancestors of already reportd MeYVMV. In
multiple alignment the comparison of the IR sequence of MeYVMV
(DNA-A) with that of other isolates of the species showed that it
has a stem-loop structure (with a nonanucleotide TAATATTAC) and
TATA box forming part of Rep promoter like other begomoviruses.
There are three iterons (ACCGA/CT), F1 and F2 on virion-sense
strand and R1 on complementary strand.
General
Imprint: |
Lap Lambert Academic Publishing
|
Country of origin: |
Germany |
Release date: |
April 2012 |
First published: |
April 2012 |
Authors: |
Fasiha Qurashi
• Saleem Haider
• Muhammad Shafiq
|
Dimensions: |
229 x 152 x 4mm (L x W x T) |
Format: |
Paperback - Trade
|
Pages: |
68 |
ISBN-13: |
978-3-8484-9934-2 |
Categories: |
Books
|
LSN: |
3-8484-9934-7 |
Barcode: |
9783848499342 |
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