THE METHODS of BACTERIOLOGICAL INVESTIGATION. - 1886 - AUTHORS
PREFACE. - URGED by the wish of my highly esteemed teacher, the
Geheimrath Koch, I have attempted in the following work to meet the
lack of a comprehensive representation of the met hods of
bacteria-investigation. It was my endeavor, as an historical and
experimental critic, to sift the whole of the literature, which was
extraordinarily scattered and in part very difficult of access, and
to select the good from the hardly conceivable confusion of useful
and use1 ess communications, in order to give to the independent
investigator a useful hand-book, and to the beginner a trnstworthy
introduction into this territory - TRANSLATORS PREFACE - THIS
translation of Hueppes excellent work on the Methods of
Bacteriological In estigat ion w as suggested by the want in
English of a satisfactory text-book on this subject for the use of
students working under my direction in the Carnegie Laboratory. The
preparation of the original was undertaken by Dr. Hueppe, at the
request of Prof. Robert Koch, and the work has been thoroughly and
care fully done. It shows a complete familiarity with the subject
in hand, is comprehensive in character, and treats carefully aUpf
the approved methods of investigation. Some difficulties have been
met in the transla tion which those acquainted with this kind of
work mill readily understand. It is literal so far as is consistent
with clearness, and no attempt has been made to attain elegance in
style or diction but it is well known that many German terms have
no exact English equivalent, and can only be rendered accurately by
a roundabout expression. This is well illustrated by 31assenkultur
as translab edby quantity-culture on page 101 et seq., which means
a culture, whether pure or not, where zt great quantity or bulk of
bacteria are growing but, since this expression can not be inserted
each time in the text, quantity-cuZtu e w ill be used wherever it
occurs. The labor of prepamring the translation was performed in
the short intervals of leisure found in the midst of numerous other
duties, and was unavoidably interrupted just before its completion,
so that some errors may be found in the book, but I think that none
of serious importance have escaped correction. The author has
requested me to emphasize the fact that the short, concise form
chosen in the work is based on an extensive historical and ex
perimentztl review of the whole subject, and that if any methods,
still much used, have been omitted or only briefly considered, it
is because they have not now the significance or importance that
has been ascribed to them by other rriters. I here desire to
aclmowledge my indebtedness to Dr. L. TV. IIubbard and Dr. S. N.
Nelson, both of New York, for valuable assistance kindly ren dered
me in the translation. The morlr has been very favornbly received
in Germany, and if this translation meets only a smn, ll part of
the same consideration in America, I shall feel well repaid for the
labor expended. CARXEGI L E A BORATOR hT Y s, m Y o a, December l,
1885. CONTENTS. PAGl INTRODUO . T IO . N . . . . . . . . 9 True
Saprophytic Forms . . . . . . . 11 1. SPOXTANEOUGBE XERATIO A N N D
TIKE PRINOIPL O E F B S TERIL IZATION . . . . 0 . . . g 16 11. FORM
O B F BA TERI A A N D MIOROSOOPIO T A E L C HNIQUE . . 28 A. The
True Endospore Bacteria . . . . ...
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