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Books > Science & Mathematics > Biology, life sciences > Biochemistry > Enzymology
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Single Molecule Enzymology - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2011)
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Single Molecule Enzymology - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2011)
Series: Methods in Molecular Biology, 778
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The last fifteen years have witnessed the birth and maturation of
many original methods and the development of protocols specific to
single molecule measurements and their analysis, including
techniques involving optical imaging, electron microscopy, optical
and magnetic trapping, and developments in atomic force microscopy.
In Single Molecule Enzymology: Methods and Protocols, experts in
the field provide procedures which enable the extraction of
detailed information about enzyme work cycles, their static and
kinetic properties, and information about their location and
activity within cells. The detailed volume offers practical advice
on many aspects of single molecule enzymology and includes
strategic overviews of interconnected methods involved in sample
preparation, single molecule measurements, and data analysis.
Written in the highly successful Methods in Molecular Biology (TM)
series format, chapters contain introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Authoritative and
up-to-date, Single Molecule Enzymology: Methods and Protocols is
intended for use within the diverse community of molecular
biologists, biochemists, and biophysicists studying enzymes in
detail and can be used by researchers planning their first single
molecule study or to aid more experienced researchers in further
developing their existing studies.
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