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Books > Science & Mathematics > Biology, life sciences > Biochemistry > Proteins
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Electron Crystallography of Soluble and Membrane Proteins - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2013)
Loot Price: R5,027
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Electron Crystallography of Soluble and Membrane Proteins - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2013)
Series: Methods in Molecular Biology, 955
Expected to ship within 10 - 15 working days
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The basic principle of electron crystallography is to calculate a
3D density map by combining the amplitudes obtained from electron
diffraction patterns with the experimental phases calculated from
images of two-dimensional crystals of membrane or soluble proteins.
This technology is very well developed and has produced a number of
atomic models of membrane proteins in a lipid environment. Focused
on comprehensive experimental protocols, Electron Crystallography
of Soluble and Membrane Proteins: Methods and Protocols covers the
entire range of techniques used in electron crystallography,
including protein sample preparation, 2D crystallization, and
screening in negative stain over electron cryo-microscopy (cryo-EM)
and data processing, as well as modeling of conformational changes.
Additional chapters provide perspective on past, present, and
future challenges as well as complementary methods. Written for the
popular Methods in Molecular Biology (TM) series, the work contains
the kind of detailed descriptions and implementation advice
necessary to ensure successful results. Comprehensive and
cutting-edge, Electron Crystallography of Soluble and Membrane
Proteins: Methods and Protocols serves laboratories new to the
methods as well as state-of-the-art facilities pursuing this
exciting area of protein science.
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