This detailed volume explores protocols for the production of
membrane proteins in a panel of heterologous organisms for
structural studies. Beginning with techniques using E. coli as a
host for the overproduction and purification of membrane proteins,
the book continues with chapters covering mammalian membrane
protein production in yeast, insect cells, mammalian cells, as well
as using virus like particles and acellular systems. Additionally,
new detergents and alternatives to detergents allowing membrane
protein purification for structural analyses are described. The
book closes with a chapter exploring the use of microscale
thermophoresis (MST) to evaluate the binding activity of
heterologously expressed proteins directly in crude membrane
extracts. Written for the highly successful Methods in Molecular
Biology series, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls.Â
Authoritative and up-to-date, Heterologous Expression of Membrane
Proteins: Methods and Protocols, Third Edition serves as an ideal
guide for scientists aiming to produce and purify functional
recombinant membrane proteins for structural studies.
General
Imprint: |
Springer-Verlag New York
|
Country of origin: |
United States |
Series: |
Methods in Molecular Biology, 2507 |
Release date: |
July 2023 |
First published: |
2022 |
Editors: |
Isabelle Mus-Veteau
|
Dimensions: |
254 x 178mm (L x W) |
Pages: |
467 |
Edition: |
3rd ed. 2022 |
ISBN-13: |
978-1-07-162370-1 |
Categories: |
Books
|
LSN: |
1-07-162370-2 |
Barcode: |
9781071623701 |
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