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Books > Medicine > Pre-clinical medicine: basic sciences > Medical genetics
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Gene Manipulation in Plant Improvement - 16th Stadler Genetics Symposium (Paperback, Softcover reprint of the original 1st ed. 1984)
Loot Price: R2,484
Discovery Miles 24 840
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Gene Manipulation in Plant Improvement - 16th Stadler Genetics Symposium (Paperback, Softcover reprint of the original 1st ed. 1984)
Series: Stadler Genetics Symposia Series
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The results obtained to date involving the use of in ~ methods to
facilitate wide hybridization in plants are voluminous and
impressive. The techniques of embryo culture, ovule culture, and
in~ pollination and fertilization represent an extension of the
normal sexual hybridization process. Successes recorded in
obtaining hybrids stem largely from circumventing prezygotic or
postzygotic hybridization barriers. Numerous recent successful
hybridizations were possible because of the development of improved
tissue and cell culture systems for crop plants and attention given
to genotypes used in hybridization attempts. Interspecific and
intergeneric hybridization utilizing the process of protoplast
fusion will bypass the limits set by all sexual me'thods. In
addition to combining complete genomes from two different species
through protoplast fusion, this system affords unique opportunities
for creating novel cytoplasmic combinations, transfer of individual
chromosomes, transfer of cytoplasmic organelles, manipulation of
male sterility, and for single gene transfer. Some caution must be
noted with regard to the extent of hybridization possible between
distantly related species. Although practically no limit exists to
the physical fusion of protoplasts from widely divergent species,
the restrictions imposed by somatic incompatibility have not been
adequately addressed. Regeneration of plants from the protoplast or
single heterokaryon level is still a major hurdle for many
important crop species before somatic cell fusion can be exploited
to produce interspecific and intergeneric hybrids. Identification
and selection of hybrids is also a limitation to the efficient
application of cell fusion methods.
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