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Differentiation of Protoplasts and of Transformed Plant Cells (Paperback, Softcover reprint of the original 1st ed. 1986)
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Differentiation of Protoplasts and of Transformed Plant Cells (Paperback, Softcover reprint of the original 1st ed. 1986)
Series: Results and Problems in Cell Differentiation, 12
Expected to ship within 10 - 15 working days
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H. BINDING and J. REINERT In collaboration with the first authors
of this volume This volume is devoted to the development of cell
clones and plants from ma nipulated cells: isolated protoplasts,
cell fusion bodies, and transformed cells. Isolated protoplasts
represent cells which are liberated from their walls and separated
from the differentiation pattern of the organism. Investigations on
re generation from protoplasts provide a better understanding of
the process and control of developmental pathways. Whereas
protoplast isolation results in alteration of the state of
differentiation of a cell, protoplast fusion is a means for the
creation of cells with novel genetic constitution. Fascinating
features are (1) to hybridize cells which-unlike gametes -did not
derive from meiosis products, (2) to bring together foreign
plastids and mitochondria and to investigate their parasexual
reactions, and (3) to match ge netic traits which had been
separated for long periods of evolution. Highly sophisticated
techniques have already been elaborated for the transfer of genes
by the use of isolated DNA and gene transfer systems. Highly
promising results have already been obtained by the use of Ti
plasmids of Agrobacterium, but direct DNA transformation is also
proving to be useful. Most of the results in these areas are
preliminary and/or limited to a few sys tem.lt is the aim of this
volume to present the main features, but at the same time to draw
attention to problems and perspectives of protoplast regeneration
and so matic cell genetics in order to stimulate further
investigations."
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