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Enzymatic Analysis - A Practical Guide (Hardcover, 2nd Revised edition)
Loot Price: R6,118
Discovery Miles 61 180
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Enzymatic Analysis - A Practical Guide (Hardcover, 2nd Revised edition)
Series: Biological Methods
Expected to ship within 10 - 15 working days
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Enzymatic Analysis: A Practical Guide is a multipurpose manual of
laboratory methods. It offers a systematic scheme for the analysis
of biological materials from the level of the whole organ down to
the single cell and beyond. It is intended as a guide to the
development of new methods, to the refinement of old ones, and to
the adaptation in general of methods to almost any scale of
sensitivity. As some may realize, the book is a sequel to A
Flexible System of Enzymatic Analysis, originally published in
1972. The major changes, other than an appropriate interchange of
authors, consist of a wholly new chapter of methods and protocols
for measuring enzymes, the addition of 13 new entries in the
metabolite chapter, and a much superior chapter on enzymatic
cycling. With considerable nostalgia, we have switched from DPN and
TPN to NAD and NADP nomenclature, which no doubt will make Otto
Warburg turn over in his grave. The incentives for the methodology
in this book came from the rigorous demands of quantitative
histochemistry and cytochemistry. These demands are specificity,
simplicity, flexibility, and, of course, sensitivity-all likewise
desirable attributes of methods for other purposes. The specificity
is provided by the use of enzyme methods. Simplicity is achieved by
leading all reactions to a final pyridine nucleotide step.
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