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Books > Science & Mathematics > Biology, life sciences > Biochemistry > Proteins
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Membrane Protein Structure and Function Characterization - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2017)
Loot Price: R4,282
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Membrane Protein Structure and Function Characterization - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2017)
Series: Methods in Molecular Biology, 1635
Expected to ship within 10 - 15 working days
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In this present volume, different approaches are detailed to
produce membrane proteins, purify them, study their function,
determine their structure, and model them in membrane. Since every
membrane protein behaves mostly in a unique way /fashion, knowledge
of guidelines and tricks may help to increase chances to express,
purify and characterize a peculiar membrane protein. Production of
correctly folded protein remains a challenge. Moreover, getting a
functional and stable protein requires to optimize membrane
mimicking environments that can be detergent or artificial
membranes. In some cases, the finding of the correct ligand which
will stabilize the desired conformation is needed. In other cases,
stabilization can be obtained using specific antibodies. This
volume also presents different techniques to analyze the functional
status of membrane proteins. Written in the highly successful
Methods in Molecular Biology series format, chapters in Membrane
Protein Structure and Function Characterization: Methods and
Protocols provide different techniques to analyze the functional
and structural status of membrane proteins. Chapters include
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
laboratory protocols, and tips on troubleshooting and avoiding
known pitfalls. Authoritative and practical, Membrane Protein
Structure and Function Characterization: Methods and Protocols aims
to ensure successful results in the further study of this vital
field.
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