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Evaluation of WET Testing as an Indicator of Aquatic Health in Effluent-Dominated Streams - A Pilot Study (Paperback, New)
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Evaluation of WET Testing as an Indicator of Aquatic Health in Effluent-Dominated Streams - A Pilot Study (Paperback, New)
Series: WERF Research Report Series
Expected to ship within 12 - 17 working days
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This project evaluated the quality of data needed to determine
relationships between chronic Whole Effluent Toxicity (WET) test
results and in-stream biological condition. A data quality
objectives approach was used, which included several proposed
measurement quality objectives (MQOs) that specified desired
precision, bias, and sensitivity of methods used. Six facilities
(four eastern and two western U.S.) participated in this study, all
having design effluent concentrations > 60% of the stream flow.
In accordance with a Quality Assurance Project Plan most of the
facilities completed four quarters of chronic Ceriodaphnia dubia,
and Pimephales promelas (fathead minnow) WET tests, and three
quarters of Selenastrum capricornutum (green algae) WET testing
following the most recent USEPA methods. Several other WET tests
were conducted to address MQOs including splits, duplicates, and
blind positive and negative controls. Macroinvertebrate, fish, and
periphyton bioassessments were conducted at multiple locations up
and downstream of each facility following the most recent USEPA
Office of Water bioassessment protocols. Test acceptance criteria
were met for most WET tests, however, this study demonstrated the
need to incorporate other MQOs in a full study (such as minimum and
maximum percent significant differences and performance on blind
samples) to ensure accurate interpretation of effluent toxicity.
More false positives, lower test endpoint (i.e., higher toxicity),
and more "failed" (non-compliant) tests were observed using No
Observed Effect Concentrations (NOEC) as compared to IC25s
(concentration causing >= 25% decrease in organism response
compared to controls). Algae tests often yielded the most effluent
toxicity in this study, however, this test was most susceptible to
false positives and high inter-laboratory variability. WET test
results exhibited few relationships with bioassessment results,
even when incorporating actual effluent dilution. Neither frequency
of WET non-compliance nor magnitude of WET were clearly related to
differences in biological condition up and downstream of a
discharge for the most part. Macroinvertebrate assessments were
most able to discriminate small changes downstream of the effluent,
followed by periphyton and then fish. The sampling methods used
were robust but a full study should collect more field replicates
up and downstream of each discharge to increase detection power.
Macroinvertebrate and periphyton assessments together appeared to
be sufficient to address project objectives. Fish assessments could
be useful as well but would entail more effort and cost per site
than expended in this project, to be useful.
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