The Enzyme Linked Immunosorbent Assay (ELISA) continues, as
predicted, to be the predominant assay used in biomedical and
agricultural research. While little has changed in the principles
of the ELISAs used since the publication of The ELISA Guidebook,
many technological advantages, including the increasing number of
reagents available for exploitation and the larger spectrum of
instruments to measure the coloured products of ELISAs, have
greatly benefited the field. Other testing technologies using
molecular techniques, predominantly the conventional and more and
more often, real time PCR, are rapidly evolving to help diagnose
and differentiate disease agents and identify substances present in
exquisitely small amounts. The ELISA is still needed either alone
in mass screening, such as serological assays, or used in tandem
with molecular techniques to fully evaluate the molecular and
antigenic nature of analytes.
John R. Crowther provides today s premier practical guide to the
understanding and application of ELISA. This Edition enlarges on
charting methods for assessing the indirect ELISA, ruggedness and
robustness of tests, aspects of kit use and validation, and
internal quality control and external quality management of data.
The author describes each method in great detail to ensure
experimental success and includes advice on equipment choice,
maintenance, and calibration. Wherever possible, helpful written
explanations are provided along with copious diagrams.
With its numerous worked examples, detailed instructions, and
extensive illustrations, The ELISA Guidebook, Second Edition offers
a powerful synthesis of all the basic concepts and practical
experimental details investigators need to understand, develop, and
apply ELISA methodology successfully in day-to-day basic and
clinical research.
General
Imprint: |
HumanaPress
|
Country of origin: |
United States |
Series: |
Methods in Molecular Biology, 516 |
Release date: |
December 2008 |
First published: |
2009 |
Authors: |
John R. Crowther
|
Dimensions: |
276 x 203 x 44mm (L x W x T) |
Format: |
Hardcover
|
Pages: |
566 |
Edition: |
2nd ed. 2009 |
ISBN-13: |
978-1-60327-253-7 |
Categories: |
Books >
Science & Mathematics >
Biology, life sciences >
Molecular biology
|
LSN: |
1-60327-253-4 |
Barcode: |
9781603272537 |
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