Mitochondrial Intoxication explores the effects toxic molecules can have upon mitochondrial physiology in the human body. Each chapter is dedicated to a specific toxicant, including pollutants, food additives, illicit and pharmaceutical drugs, and heavy metals. This book considers the implications and impact these have upon mitochondria and the diseases that can result from dysfunction and impairment in the human body. Furthermore, the book provides an overview of mitochondrial physiology and assesses the advances and challenges in testing mitochondrial toxicity. Case studies exploring mitochondrial intoxication in pregnancy and in the geriatric population are also included. This is a comprehensive reference on the main toxicants impacting mitochondria in the human body, and the consequences this can have for health and disease.

Analytical Techniques in Biosciences: From Basics to Applications presents comprehensive and up-to-date information on the various analytical techniques obtainable in bioscience research laboratories across the world. This book contains chapters that discuss the basic bioanalytical protocols and sample preparation guidelines. Commonly encountered analytical techniques, their working principles, and applications were presented. Techniques, considered in this book, include centrifugation techniques, electrophoretic techniques, chromatography, titrimetry, spectrometry, and hyphenated techniques. Subsequent chapters emphasize molecular weight determination and electroanalytical techniques, biosensors, and enzyme assay protocols. Other chapters detail microbial techniques, statistical methods, computational modeling, and immunology and immunochemistry. The book draws from experts from key institutions around the globe, who have simplified the chapters in a way that will be useful to early-stage researchers as well as advanced scientists. It is also carefully structured and integrated sequentially to aid flow, consistency, and continuity. This is a must-have reference for graduate students and researchers in the field of biosciences.

Encyclopedia of Biological Chemistry, Six Volume Set has always been characterized by its unique and comprehensive content. Since publication of the 2nd edition, many important discoveries have been made leading to novel concepts in several areas of biochemistry, and new technologies have advanced our understanding of key processes of life. All of these advances are included in the new and expanded third edition. With its 6 volumes, this is the most up-to-date and complete resource on biochemistry and molecular biology, provided through contributions by leading experts in the field.

Handbook of Hormones: Comparative Endocrinology for Basic and Clinical Research, Second Edition presents a catalog of fundamental information on the structure and function of hormones from basic biology to clinical use, offering a rapid way to obtain specific facts about the chemical and molecular characteristics of hormones, their receptors, signaling pathways, and the biological activities they regulate. The book's stellar editorial board, affiliated with the Japan Society for Comparative Endocrinology, brings together authors that present a compelling structure of each hormone with a consistent presentation that provides a primer surrounding the plethora of hormones that now exist. Comparative endocrinology continues to rapidly expand and new information about hormones is being produced almost daily, making it important to stay up-to-date. Hormone, paracrine, and autocrine factors have been identified as key players in a range of different systems, including immune, musculoskeletal and cardiovascular. Frontiers between disciplines are being blurred and many scientists in fields other than endocrinology are interested in hormones. Scientists now have the unprecedented opportunity to look from invertebrates to vertebrate and identify novel regulatory factors and understand their function and how they determine an organism's physiology and survival.

The Human Mitochondrial Genome: From Basic Biology to Disease offers a comprehensive, up-to-date examination of human mitochondrial genomics, connecting basic research to translational medicine across a range of disease types. Here, international experts discuss the essential biology of human mitochondrial DNA (mtDNA), including its maintenance, repair, segregation, and heredity. Furthermore, mtDNA evolution and exploitation, mutations, methods, and models for functional studies of mtDNA are dealt with. Disease discussion is accompanied by approaches for treatment strategies, with disease areas discussed including cancer, neurodegenerative, age-related, mtDNA depletion, deletion, and point mutation diseases. Nucleosides supplementation, mitoTALENs, and mitoZNF nucleases are among the therapeutic approaches examined in-depth. With increasing funding for mtDNA studies, many clinicians and clinician scientists are turning their attention to mtDNA disease association. This book provides the tools and background knowledge required to perform new, impactful research in this exciting space, from distinguishing a haplogroup-defining variant or disease-related mutation to exploring emerging therapeutic pathways.

High-Density Sequencing Applications in Microbial Molecular Genetics, Volume 612 in the Methods of Enzymology series provides the latest on the high-density sequencing of DNA and cDNA libraries and how they have revolutionized contemporary research in biology. Methods permitting tens of millions of sequence reads in a single experiment have paved the way to genome-wide studies that are contributing to our understanding of the complexity of living systems. Chapters in this updated volume include Characterizing the role of exoribonucleases in the control of microbial gene expression: Differential RNA seq., Conformational studies of bacterial chromosomes by high-throughput sequencing methods, Measuring mRNA degradation, and more. Addition sections cover Global recognition patterns of bacterial RNA-binding proteins, High-resolution profiling of NMD targets, and the Generation of a metagenomic 3C/Hi-C library of human gut microbiota, Genome-wide mapping of yeast retrotransposons integration target sites, Measuring protein synthesis rates, Finding unsuspected partners of small RNAs with new screening approaches, Use of multiplexed transcriptomics to define the relationship between promoter sequence and transcription output, RNA-based control of quorum sensing in Vibrio cholerae, amongst other highly regarded topics.

Enzymes of Energy Technology, Volume 613 in the Methods in Enzymology series, highlights new advances in the field, with this updated volume presenting interesting chapters written by an international board of authors. Chapters include Purification of fully active and crystallizable photosystem II from thermophilic cyanobacteria, Production and manipulation of [NiFeSe]-hydrogenases for renewable hydrogen research, Hydrogen production by [FeFe]-hydrogenases, Production and properties of enzymes that activate and produce carbon monoxide, Recombinant [NiFe]-hydrogenases from E. coli, Working with nitrogenase, Oxygen--tolerant [NiFe]-hydrogenases, Cytoplasmic and Membrane Bound Hydrogenases from the hyperthermophile Pyrococcus furiosus, and more. Additional sections cover Enzymatic conversion of methane into useful chemicals, Production and investigations of trans-membrane electron transfer protein, Characterization of post-translational modifications in methyl-coenzyme M reductase in diverse methanogens by mass-spectrometry, Reductive activation of carbon dioxide by formate dehydrogenases, and Lytic polysaccharide monooxygenases in biofuel processing. 14. Production and manipulation of blue copper oxidases for technological applications Yasmina Mekmouche

Progress in Molecular Biology and Translational Science, Volume 160 provides the most topical, informative and exciting monographs available on a wide variety of research topics related to prions, viruses, bacteria and eukaryotes. The series gives in-depth knowledge on the important molecular biological aspects of organismal physiology and function, along with insights on how this knowledge can be applied to understand and ameliorate human disease. This updated release covers Clinical Drugs in the Environment, Group I Intron Trans-Splicing, Adult Stem Cells on Regenerative Therapy, CRISPR in Animals and Animal Models, The Role of 3'-5'Exoribonucleases in RNA Degradation, and more.

Modern Approaches in Drug Discovery, Volume 611, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as Target Identification and Validation, Cell Painting/High Content Imaging, Target ID using chemical probes, Mining the microbiome for targets, Data driven approaches for diversity and drug-likeness, Affinity-based screening, Fragment screening (X-ray), Array-based approaches, Hit-to-lead: assessment and improvement of drug-like properties, Hit assessment and prioritization, Lead Optimization: fine tuning and risk mitigation, and more.

Biology of T Cells: Part A, Volume 341, the latest release in the International Review of Cell and Molecular Biology, reviews and details current advances in cell and molecular biology. The IRCMB series maintains the highest standard by publishing timely topics authored by prominent cell and molecular biologists. Specialized topics in this release include TCR signaling: Molecules and mechanisms, TCR diversity: Purpose and generation, Transcriptional programs underlying T-cell differentiation and function, Surface phenotypes of CD8+ and CD4+ T cells, Co-stimulation and co-inhibition in CD8+ and CD4+ T cells, Regulated cell death and T cells, Molecular mechanisms behind T-cell priming by DCs, and more.

Darwin's Pangenesis and its Rediscovery Part B explores Darwin's Pangenesis, an expanded cell theory and unified theory of heredity and variation from over 150 years ago that strengthened his theory of evolution and explained many phenomena of life. Now, new discoveries on circulating DNA, mobile RNAs, prions and extracellular vesicles are providing striking evidence for the chemical existence of Darwin's imaginary gemmules. In addition, new evidence for the inheritance of acquired characters, graft hybridization, and many other phenomena that Pangenesis supposedly explains are progressing, and are hence explored in this comprehensive volume. Specific chapters in this new volume include Darwin and Mendel: The Historical Connection, Darwin's Pangenesis and Graft Hybridization, Darwin's Pangenesis and Medical Genetics, Darwin's Pangenesis and Certain Anomalous Phenomena, and Natural Selection and Pangenesis: The Darwinian Synthesis.

Neuroepigenetics and Mental Illness, Volume 158, the latest release in the Progress in Molecular Biology and Translational Science series, seeks to provide the most topical, informative, and exciting monographs available on a wide variety of research topics related to prions, viruses, bacteria and eukaryotes. The series seeks to provide readers with in-depth knowledge of important molecular biological aspects of organismal physiology and function, with this release focusing on Neuroepigenetics in mental illness, Neuroepigenetics of development and neurodevelopmental disorder, Neuroepigenetics of aging and age-related neurodegenerative disorders, Neuroepigenetics of prenatal psychological stress, Neuroepigenetics of the HPA axis, Neuroepigenetics of the serotonergic system, and more.

Microfluidics in Cell Biology Part A: Volume 146, the latest release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are sections on Cell monolayers/spheroids, Collective migration in microtubes, Leukocyte adhesion dynamics on endothelial monolayers under flow, Constrained spheroid for perfusion culture, Cells in droplet arrays, Heart on chips, Kidney on chips, Liver on chips and hepatic immune responses, Gut on chips, 3D microvascular model-based lymphoma model, Blood brain barrier on chips, Multi-organ-on-a-chip for pharmacokinetic analysis, Cancer immunotherapy on chips, and more.

G Protein-Coupled Receptors: Emerging Paradigms in Activation, Signaling and Regulation, Part A, Volume 337 in the International Review of Cell and Molecular Biology series, reviews and details current advances in cell and molecular biology. The IRCMB series has a worldwide readership, maintaining a high standard by publishing invited articles on important and timely topics that are authored by prominent cell and molecular biologists. This new release includes comprehensive sections on the classical paradigms of GCPR signaling and regulation, the family of small GTPases: Beyond heterotrimeric G proteins, GPCR endocytosis: Arrestins, arrestin-like proteins, signaling from internalized G Protein-Coupled Receptors, GPCR kinases, and more.

Therapeutic Proteins and Peptides, Volume 112 in an ongoing series promotes further research in the discovery of new therapeutic targets that can be affected by therapeutic proteins and peptides to cure or manage symptoms of human diseases, with this release focusing on the Rational Design of Stable Liquid Formulations of Biopharmaceuticals, Formulation strategies for peptides, proteins and antibodies using nanotechnology, the Solution structural dynamics of therapeutic peptides and their adsorption on plasmonic nanoparticles, Enzymatic approaches of protein-polymer conjugation, Chimeric small antibody fragments as a strategy to deliver therapeutic payloads, Smart cell-penetrating peptide-based techniques for cytoplasmic delivery of therapeutic macromolecules, and more.

Chemical and Biochemical Approaches for the Study of Anesthetic Function, Part B, Volume 603, presents a coherent description of the campaign towards understanding anesthesia. It includes a variety of highly debated topics, including sections on computational approaches, best practices for simulating ligand-gated ion channels interacting with general anesthetics, computational approaches for studying voltage-gated ion channels modulation by general anesthetics, anesthetic parameterization, the kinetic modeling of electrophysiology data, evolving biophysical technologies, fluorescent anesthetics, lipids, membranes and pressure reversal, in vivo technologies, and more.

Sirtuins in Health and Disease, Volume 154 presents the reactions catalyzed by sirtuins in terms of their unique coenzyme NAD+-dependent catalytic mechanisms, the ways to elucidate these mechanisms, and the design of the inhibitory compounds against the sirtuin-catalyzed reactions as potential therapeutic agents for human diseases. Sections cover the concepts and chemical tools in sirtuin research, SIRT4 and SIRT7, the regulatory effects of NAD metabolic pathways on sirtuin activity, sirtuins as modifiers of Huntington's Disease (HD) pathology, parasite sirtuins as targets for novel chemotherapeutic agents, targeting sirtuins - substrate specificity and inhibitor design, and chemical probes in sirtuin research. This book focuses on the outstanding issues in the sirtuin field and implications for future sirtuin research.

International Review of Cell and Molecular Biology. Volume 336 reviews and details current advances in cell and molecular biology. The IRCMB series has a worldwide readership, maintaining a high standard by publishing invited articles on important and timely topics that are authored by prominent cell and molecular biologists. This updated volume includes specific chapters on Membrane trafficking in autophagy, Targeting the metabolism of leukemic cells, Autophagic flux in Alzheimer's disease, Transcriptional alterations associated with celiac disease, and the Regulation of antitumor immunity by IDO1, amongst other timely topics. The articles published in IRCMB have a high impact and an average cited half-life of 9 years. This great resource ranks high amongst scientific journals dealing with cell biology.

WNT Signaling, Volume 153, the latest release in the Progress in Molecular Biology and Translational Science series, seeks to provide the most topical, informative, and exciting monographs available on a wide variety of research topics related to prions, viruses, bacteria and eukaryotes. The series provides readers with in-depth knowledge of important molecular biological aspects of organismal physiology and function, along with insights into how this knowledge may be applied to understand and ameliorate human disease. Each volume is edited by an internationally recognized expert who selects contributors at the forefront of a field.

Enzymes as Sensors, Volume 589, the latest release in the Methods in Enzymology series, covers a variety of topics, including advances in genetically coded fluorescent sensors, enzymes as sensors, and bioapplications of electrochemical sensors and biosensors. Users will find a comprehensive discussion of timely topics that presents a micro-level delivery of specific content related to the study of enzymes in sensors. New to this edition are highly specialized chapters on integrated strategies for gaining a systems level view of dynamic signaling networks, sensitive protein detection and quantification in paper-based microfluidics for point-of-care, and microneedle enzyme sensor arrays for continuous in vivo monitoring. This state-of-the-art series is ideal for those interested in the latest information on enzymology, with this edition focusing on sensors and their role in enzymes.

International Review of Cell and Molecular Biology reviews and details current advances in cell and molecular biology. This most current release in this highly cited series focuses on such timely topics as novel treatments in immunotherapy in breast cancer, chemokine receptor signaling and the hallmarks of cancer, tumor-targeting antibodies, and a section on mitochondrion and whether they are a common organelle for cell death. As always, readers will find the articles in this series to be of great value, with a high impact and average cited half-life of nine years. The IRCMB series has a worldwide readership, maintaining a high standard by publishing invited articles on important and timely topics that are authored by prominent cell and molecular biologists. This great resource ranks high amongst scientific journals dealing with cell biology.

Hematopoietic Stem Cell Niche focuses on an individual organ, looking at the stem cells in the organ itself (if they exist), their niches, and how to use them alongside relevant methods and protocols. This series addresses stem cells during development, homeostasis, and disease/injury of the respective organs, presenting new developments, such as new data on disease and clinical applications. Specific chapters delve into the evolution of hematopoietic stem cell niches, the role of CNS in the regulation of HSCs, and targeting the bone marrow niche in hematological malignancies. In addition, video content illustrates protocols, transplantation techniques, and work with mice.

Chromatin Proteins and Transcription Factors as Therapeutic Targets, the latest volume in the Advances in Protein Chemistry and Structural Biology series is an essential resource for protein chemists. Each volume brings forth new information about protocols and analysis of proteins, with each thematically organized volume guest edited by leading experts in a broad range of protein-related topics.

Neurotrophins, the latest volume in the Vitamins and Hormones series first published in 1943, and the longest-running serial published by Academic Press, provides up-to-date information on crystal structures and basic structural studies on neurotrophins and their receptors, neurotrophin functions, and the biological actions of neurotrophins related to clinical conditions and disease. Each volume is thoroughly reviewed and focuses on a single molecule or disease that is related to vitamins or hormones, with the topic broadly interpreted to include related substances such as transmitters, cytokines, growth factors, and others.

Fly Models of Human Diseases provides users with a comprehensive survey on fly models of human diseases in the field of developmental biology. It is ideal for researchers in animal and plant development, and for students and professionals working in a variety of fields related to the topic.