Three-Dimensional Electron Microscopy, Volume 152 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters focusing on FIB-SEM of mouse nervous tissue: fast and slow sample preparation, Serial-section electron microscopy using ATUM - Automated Tape collecting Ultra-Microtome, Software for automated acquisition of electron tomography tilt series, Scanning electron tomography of biological samples embedded in plastic, Cryo-STEM tomography for Biology, CryoCARE: Content-aware denoising of cryo-EM images and tomograms using artificial neural networks, Expedited large-volume 3-D SEM workflows for comparative vertebrate microanatomical imaging, and many other interesting topics.

The objective of this book is to bring into focus the practical application of the theoretical knowledge of crop physiology. This book on Crop Physiology describes basic principles and procedures with exercises dealing with demonstration of important physiological and metabolic processes viz. osmosis, diffusion, water potential, stomatal frequency and index, plant water status, water relation, Measurement of absorption spectrum of chloroplastic pigments and fluorescence, leaf area, plant growth and transpiration. Demonstration of photosynthesis, transpiration, relationship between absorption and transpiration, conditions for seed germination, seed viability and dormancy aspects, chemical solution preparation for physiological and biochemical analysis of plant samples.

RNA Recognition, Volume 623, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This updated volume covers a variety of topics, including The Preparation of cooperative RNA recognition complexes for crystallographic structural studies, Methods for thermal denaturation studies of fluorogenic aptamers, Dynamic combinatorial chemistry as a rapid, fragment-based approach to RNA-targeted compound discovery, Using a click chemistry assay to identify natural product ligands for pre-microRNAs, Lessons from exploration of chemical and structural small molecule:RNA space, Using ligand-observed NMR to study RNA-small molecule interactions, and much more.

Membrane Transporters in the Pathogenesis of Cardiovascular and Lung Disorders, Volume 83, the latest release in the Current Topics in Membranes series, highlights new advances in the field, with this volume presenting chapters from recognized experts on topics such as cardiotonic steroids, Na+, K+ pumps and vascular fibrosis, purinergic signaling in the lung, structural models of a2-subunit N-termini and binding interfaces, ubiquitous and cell type-specific transcriptomic changes triggered by dissipation of monovalent cation gradients, the Na, K-ATPase alpha2 isoform in cardiovascular pathologies, the role of cell swelling and volume-sensitive ion channels in stroke pathology, structure-function relationships in the renal NaCl cotransporter, and more.

Chemical and Synthetic Biology Approaches To Understand Cellular Functions - Part B, Volume 622, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting chapters that cover the Design of optogenetic proteins, the Application of optogenetic proteins, Antibody aggregation mechanism probed by a fluorescently-labeled antibody with fluorescence correlation spectroscopy, Bimane labeling of B-arrestins to measure their interaction with GPCRs, Reversible biotinylation of proteins for investigating their interaction with partners, Chemical biology approaches to study RNA cytidine acetylation, Salt sensitive intein in robotic production of peptides, and much more.

The Immortal Germline, Volume 135 in the Current Topics in Developmental Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including Balbiani body, germ plasm and polarity, Wolbachia and the germline, Human germ line biology, Reproduction/immunity and sperm success, Mammalian spermatogenesis, Reproduction/regeneration and germ cells in planarians, Germline stem cell homeostasis, Germline soma interactions/sex determination, Reconstituting the mouse germ-cell specification pathway in vitro, Maternal Germ line inheritance of mitochondria, Transposon strategies, and more.

Model Ecosystems in Extreme Environments, Second Edition examines ecosystems at the most extreme habitats and their interaction with the environment, providing a key element in our understanding of the role and function of microorganisms in nature. The book highlights current topics in the field, such as biodiversity and the structure of microbial communities in extreme environments, the effects of extreme environmental conditions on microbial ecosystems, and ecological and evolutionary interactions in extreme environments, among other topics. It will be a valuable text for faculty and students working with extremophiles and/or microbial ecology and researchers, including astrobiologists, biologists, evolutionary scientists, astronomers, geochemists and oceanographers.

Nanotechnology in Modern Animal Biotechnology: Concepts and Applications discusses the advancement of nanotechnologies in almost every field, ranging from materials science, to food, forensic, agriculture and life sciences, including biotechnology and medicine. Nanotechnology is already being harnessed to address many of the key problems in animal biotechnology, with future applications covering animal biotechnology (e.g. animal nutrition, health, disease diagnosis, and drug delivery). This book provides the tools, ideas and techniques of nanoscale principles to investigate, understand and transform biological systems. Nanotechnology provides the ability to manipulate materials at atomic and molecular levels and also arrange atom-by-atom on a scale of ~1-100 nm to create, new materials and devices with fundamentally new functions and properties arising due to their small scale.

Advances in Microbial Physiology, Volume 74, the latest release in this ongoing series, continues the long tradition of topical, important, cutting-edge reviews in microbiology. The book contains updates in the field, with comprehensive chapters covering The electrifying physiology of Geobacter bacteria 30 years on, Adaptive morphogenesis in bacteria, Bacterial energetics and respiratory metabolism, Bacteria respiration during infection, Regulation of Organohalide Respiration, Bioenergetics of Campylobacter jejuni, Formate Hydrogenlyases, Bioenergetical Aspects of the Bacterial and Archaeal Hydrogen Metabolism, and more.

International Review of Cell and Molecular Biology, Volume 346, reviews and details current advances in cell and molecular biology. The IRCMB series has a worldwide readership, maintaining a high standard by publishing invited articles on important and timely topics that are authored by prominent cell and molecular biologists. Sections in this new release include the karyosphere (karyosome) and its peculiar structure of the oocyte nucleus, organoids as models of disease, lipid droplets as organelles, the dark side of apoptosis, interconnections between autophagy and secretion, and the regulation and function of intracellular pressure in cell biology.

Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part A, Volume 621, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this volume covering Site-directed ethylation of membrane proteins for measuring conformational transitions in lipid bilayers, the Design and synthesis of fluorescent activity probes for protein phosphatases, Stains, Utilizing split-nanoLuc fragments as luminescent probes for protein solubility in living cells, SH2-domain based sensor for intracellular recognition of sulfo-tyrosine, DNA-encoded immunoglobulins for detection of parasites, An engineered TEV protease - calmodulin fusion based sensor for neuronal calcium recording, and much more.

Glycans and Glycosaminoglycans as Clinical Biomarkers and Therapeutics - Part B, Volume 163 in the Progress in Molecular Biology and Translational Science series, provides informative monographs on a variety of research topics related to Glycans and glycosaminoglycans as clinical biomarkers and therapeutics. Topics in this update include an Overview of Fugal Glycan-based Therapeutics, Heparin: An Essential Drug for Modern Medicine, Low Molecular Weight Heparins and Their Clinical Applications, The Clinical Use of Fondaparinux: A Synthetic Heparin Pentasaccharid, Heparinoids as Clinically Used Drugs, Marine Glycan-Derived Therapeutics in China, Efficacy of Heparinoid PSS in Treating Cardiovascular and other Diseases-30 Years Clinical Applications in China, and more.

Diagnostic Molecular Biology describes the fundamentals of molecular biology in a clear, concise manner to aid in the comprehension of this complex subject. Each technique described in this book is explained within its conceptual framework to enhance understanding. The targeted approach covers the principles of molecular biology including the basic knowledge of nucleic acids, proteins, and genomes as well as the basic techniques and instrumentations that are often used in the field of molecular biology with detailed procedures and explanations. This book also covers the applications of the principles and techniques currently employed in the clinical laboratory.

Vertebrate Skeletal Development, Volume 133, the latest release in the Current Topics in Developmental Biology series, presents interesting chapters on a variety of topics, with this edition focusing on Craniofacial skeletal development, Regulatory mechanism of jawbone and tooth development, Development of the axial skeleton and intervertebral discs, Stem and progenitor cells in skeletal development, Origin, functioning and morphogenetic activity of limb synovial joint, ECM signaling in cartilage development and endochondral ossification, Sox genes in skeletal development, Wnt-Signaling in Skeletal Development, Gas signaling in skeletal development and diseases, FGF signaling in skeletal development, Bone morphogenetic growth factors in bone development, and more.

The lymphatic system develops and functions in parallel with the blood circulatory system (termed the "hemovasculature") and accomplishes transport of interstitial fluids, dietary lipids, and reverse transport of cholesterol, immune cells, and antigens-providing a critical homeostatic fluid balance and transmission of immune cells and mediators back to the cardiovascular system. Although the daily flow of lymph (normally 1-2 L/day under unstressed conditions) is far lower than that of daily blood flow (which is 7,500 L/day), without the adequate functioning of the lymphatics, virtually all organs and tissues would acutely suffer many different physical and inflammatory stresses ranging from edema to organ system failure. Although blood and lymphatic vessels often form in anatomic parallels to one another, our knowledge of the workings of the lymphatic system, the fine structure of lymphatic networks, how they function in different organs, and how they are regulated physiologically and immunologically are far from parallel; our knowledge of the lymphatic system still remains at only a tiny fraction of what is understood about the cardiovascular system. Although both the cardiovascular and lymphatic systems are important transport systems, what they transport and how they transport and propel these very different cargoes could not be more dissimilar. This book provides an overview of the history of the discovery (and re-discovery) of the components of the lymphatic system, lymphatic anatomy, physiological functions of lymphatics, molecular features of the lymphatic system, and clinical perspectives involving lymphatics which may be of interest to scientists, clinicians, patients, and the lay public. We provide a current understanding of some of the more important structural similarities and differences between lymphatics and the blood vascular system, their coordinated control by angiogenic and hemangiogenic growth factors and other modulators, the fate and lineage determinants which control lymphatic development, and the roles that lymphatics may play in several different diseases.

Plant Development and Evolution, the latest release in the Current Topics in Developmental Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on the Evolution of the plant body plan, Lateral root development and its role in evolutionary adaptation, the Development of the vascular system, the Development of the shoot apical meristem and phyllotaxis, the Evolution of leaf diversity, the Evolution of regulatory networks in land plants, The role of programed cell death in plant development, the Development and evolution of inflorescence architecture, the Molecular regulation of flower development, the Pre-meiotic another development, and much more.

Atomic Force Microscopy for Nanoscale Biophysics: From Single Molecules to Living Cells summarizes the applications of atomic force microscopy for the investigation of biomolecules and cells. The book discusses the methodology of AFM-based biomedical detection, diverse biological systems, and the combination of AFM with other complementary techniques. These state-of-the-art chapters empower researchers to address biological issues through the application of atomic force microscopy. Atomic force microscopy (AFM) is a unique, multifunctional tool for investigating the structures and properties of living biological systems under aqueous conditions with unprecedented spatiotemporal resolution.

Microfluidics in Cell Biology Part C, Volume 148, a new release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are three sections on microfluidics in various multi-cellular models, including microfluidics in cell monolayers/spheroids, microfluidics in organ on chips, and microfluidics in model organisms. Specific chapters discuss collective migration in microtubes, leukocyte adhesion dynamics on endothelial monolayers under flow, constrained spheroid for perfusion culture, cells in droplet arrays, heart on chips, kidney on chips, liver on chips, and more.

Microbiology of Atypical Environments, Volume 45, presents a comprehensive reference text on the microbiological methods used to research the basic biology of microorganism in harsh, stressful and sometimes atypical environments (e.g. arctic ice, space stations, extraterrestrial environments, hot springs and magnetic environments). Chapters in this release include Biofilms in space, Methods for studying the survival of microorganisms in extraterrestrial environments, Persistence of Fungi in Atypical (Closed) Environments Based on Evidence from the International Space Station (ISS): Distribution and Significance to Human health, Methods for visualizing microorganisms in Icy environments, Measuring microbial metabolism at surface-air interfaces and nuclear waste management, amongst others.

Cancer Therapy and Diagnosis, Part B, Volume 44 in The Enzymes series highlights new advances in the field, with this new volume presenting interesting chapters on Mesoporous silica nanoparticle synthesis, Periodic mesoporous organosilica, Nanovalves and other nanomachine-equipped nanoparticles and controlled release, Two-photon light control and photodynamic therapy, Biodegradable PMO nanoparticles, Cationic mesoporous silica and protein delivery, Drug loading, stimuli-responsive delivery and cancer treatment, Animal models and cancer therapy, siRNA delivery and TWIST shutdown for ovarian cancer treatment, and TBC (mesoporous silica nanoparticles and cancer therapy or biodistribution of MSN).

Effect of High-Pressure Technologies on Enzyme: Science and Applications provides a deep, practical discussion of high-pressure processing (HPP) and high-pressure homogenization (HPH) technologies and biochemical approaches, applied across research and industry, with applications ranging from food to pharmaceuticals and commercial enzyme production. Early chapters discuss foundational aspects of HPP and HPH approaches; the science of enzyme modification; and basic aspects of enzyme activity, stability, and structure as studied in biochemical processes. Later chapters consider the effect of HPP and HPH technologies and their mechanisms of controlling enzyme modification to improve enzyme performance for chosen applications. Special attention is paid to the application of HPP and HPH technologies and enzyme modifications in food processing, microbial enzyme modification, drug discovery, and production of other commercial enzymes, as well as the challenges of undesirable enzyme inactivation. The final chapter discusses future directions of the field and technologies, and expanded applications.

Insect Molecular Genetics: An Introduction to Principles and Applications, Fourth Edition provides the most recent advances and research in entomology and molecular genetics. It demonstrates the role molecular genetics plays in pest management and basic insect biology research, assuming readers have little to no prior knowledge on the subject. This newest edition features updates on the latest trends and discoveries, such as CRISPR-Cas genome editing methods, epigenetic inheritance, DNA amplification techniques, and molecular phylogenies. Certain chapter topics, including P-element mediated transformation and insect population molecular techniques, are condensed and updated to create a more comprehensive and concise read. This foundational text is a valuable resource for entomologists with limited training in molecular genetics, and will be an ideal source for pest control advisors who need to understand the vocabulary and methods that are used to solve entomological issues. Contains a new chapter on CRISPR-CAS and the basic biology of these systems related to arthropods Includes new and updated methods and examples of insect gene modification, along with discussions of regulatory and ethical issues Offers compiled and comprehensive terminology at the introductory level Illustrated with diagrams and tables to further explain key topics and concepts

Mitochondria and Longevity, Volume 340, the latest release in the International Review of Cell and Molecular Biology series reviews and details current advances in cell and molecular biology. The IRCMB series has a worldwide readership, maintaining a high standard by publishing invited articles on important and timely topics with this release focusing on topics such as Mitochondria metabolism and aging, Mitohormesis, Mitochondrial dynamics in the aging stem cell compartment, Mitochondrial proteostasis and aging, Mitochondrial DNA mutations and aging, Mitochondrial sirtuins, NAD+, NADH and aging, Mitophagy and aging, Mitochondria, calcium transport and aging.

Advances in Stem Cells and Their Niches addresses stem cells during development, homeostasis, and disease/injury of the respective organs, presenting new developments in the field, including new data on disease and clinical applications. Video content illustrates such areas as protocols, transplantation techniques, and work with mice.