Advances in CRISPR/Cas and related technologies, Volume 179, the latest release in this ongoing series, deals with a wide variety of research topics related to recent advancement in the genome editing techniques. Associated chapters in this new release include Challenges for Therapeutic application of CRISPR Cas techniques, Mitochondrial DNA modification by CRISPR Cas System: Challenges and future direction, Trends in CRISPR Cas technology application in cancer, Modified CRISPR-Cas for next generation application, Application of CRISPR Cas in Synthetic Biology: Challenges and Scopes, History of CRISPR Cas system from bacterial Adaptive Immune System to research application, and more.

Correlative Light and Electron Microscopy IV, Volume 162, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Besides the detailed description of protocols for CLEM technologies including time-resolution, Super resolution LM and Volume EM, new chapters cover Workflow (dis)-advantages/spiderweb, Serial section LM + EM, Platinum clusters as CLEM probes, Correlative Light Electron Microscopy with a transition metal complex as a single probe, SEM-TEM-SIMS, HPF-CLEM, A new workflow for high-throughput screening of mitotic mammalian cells for electron microscopy using classic histological dyes, and more.

Pore Forming Toxins, Volume 649 in the Methods in Enzymology series continues the legacy of this premier serial with quality chapters authored by field leaders. Chapters in this new release include X-ray crystallography shines a light on pore-forming toxins, Giant unilamellar vesicles for studying activity of pore forming proteins, Combined applications of fluorescence spectroscopy and molecular dynamics simulations in studies of Diphtheria Toxin translocation domain, Biophysical approaches to study actinoporin-lipid interactions, Molecular basis for activation of Actinoporins by lipids, Engineered ClyA for detection of biological molecules, Pore-forming Toxins for the Size-Discrimination of Polymers and Biopolymers: Towards Biomolecules Sequencing, and much more.

Immunopathology of Celiac Disease, Volume 359 presents the latest release in this ongoing series on novel and widely studied aspects of celiac disease pathogenesis. Topics covered in this new volume include Omics of Celiac Disease, Implication of HLA genes in Celiac Disease, Macrophages & Dendritic Cells in Celiac Disease, Tight junction disruption in the development of celiac disease, Implication of epithelial cell dysfunction in CeD, Involvement of p31-p43 gluten peptide in the celiac disease related immune/inflammatory response, The biology of refractory celiac disease, Involvement of lncRNAs in Celiac disease pathogenesis, and more.

Advances in Genetics, Volume 107, provides the latest information on the rapidly evolving field of genetics, presenting new medical breakthroughs that are occurring as a result of advances in our knowledge of the topic. The book continually publishes important reviews of the broadest interest to geneticists and their colleagues in affiliated disciplines, with this new release including chapters on Advances in Asthma Genetics: Filling persistent gaps, Nutritional control of postembryonic development progression and arrest in Caenorhabditis elegans, Genetic determinants of climate-resilience traits in millets, Founder variants and population genomes - towards precision medicine, and much more.

Protein Kinases in Drug Discovery, Volume 124 discusses the latest information on protein kinases and how they modify other proteins by chemically adding phosphate groups to them. New chapters in this release include Transport Proteins and AMPs: Implications in Human Disease, Protein kinase CK2 inhibition as a pharmacological strategy, Emerging role of Protein kinase in diabetes mellitus: From Mechanism to therapy, Dual Roles of ATP-binding site in Protein Kinases: Orthosteric inhibition and Allosteric Regulation, Pseudokinases in drug discovery and development: progress, challenges and future prospects, Comparison of knowledge-based vs. combinatorial peptide library approaches for the identification of protein kinase substrates, and more.

Enzymatic Plastic Degradation, Volume 648 in the Methods in Enzymology series, continues the legacy of this premier serial with chapters authored by leaders in the field. Chapters in this latest release include Evaluating plastic pollution and environmental degradation, Assessment methods for microplastic pollution in the oceans and fresh water, Exploring microbial consortia from various environments for plastic degradation, Characterization of filamentous fungi for attack on synthetic polymers via biological Fenton chemistry, Synthesis of radioactive-labeled nanoplastics for assaying the environmental (microbial) PS degradation, Exploring metagenome for plastic degrading enzymes, Cutinases from thermophilic bacteria (actinomycetes): from identification to functional and structural characterization, and much more.

Expansion Microscopy for Cell Biology, Volume 161 in the Methods in Cell Biology series, compiles recent developments in expansion microscopy techniques (Pro-ExM, U-ExM, Ex-STED, X10, Ex-dSTORM, etc.) and their applications in cell biology, ranging from mitosis, centrioles or nuclear pore complex to plant cell, bacteria, Drosophila or neurons. Chapters in this new release include Protein-retention Expansion Microscopy: Improved Sub-cellular Imaging Resolution through Physical Specimen Expansion, Ultrastructure Expansion Microscopy (U-ExM), Expansion STED microscopy (ExSTED), Simple multi-color super-resolution by X10 microscopy, Expansion microscopy imaging of various neuronal structures, Mapping the neuronal cytoskeleton using expansion microscopy, Mechanical expansion microscopy, and much more.

Linkers in Biomacromolecules, Volume 647 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. New sections in this updated release include chapters devoted to ser-gly linkers + models, sergGly- EAAAK linkers, Effect of crowding on linker behavior, Protathether. Experimental approach to quickly test different linkers in fusion proteins, Linkers in enzyme fusions/cascade catalysis, Linker dependence of avidity in multivalent interactions between disordered proteins, ER/K a-helix linker, The effect of chirality and steric hindrance on intrinsic backbone conformational propensities: tools for protein design, Linker design in protein-based nanostructures, and much more. Linkers in Biomacromolecules, Volume 647 in the Methods in Enzymology series continues the legacy of this premier serial with quality chapters authored by leaders in the field. New sections in this updated release include chapters devoted to ser-gly linkers + models, sergGly- EAAAK linkers, Effect of crowding on linker behavior, Protathether. Experimental approach to quickly test different linkers in fusion proteins, Linkers in enzyme fusions/cascade catalysis, Linker dependence of avidity in multivalent interactions between disordered proteins, ER/K a-helix linker, The effect of chirality and steric hindrance on intrinsic backbone conformational propensities: tools for protein design, Linker design in protein-based nanostructures, and much more.

Molecular Biology of Neurodegenerative Diseases: Visions for the Future, Part B, Volume 177 in the Progress in Molecular Biology and Translational Science series, provides the most topical, informative and exciting monographs available on a wide variety of research topics. The series includes in-depth knowledge on the molecular biological aspects of organismal physiology, with this release including chapters on Alzheimer's disease, Prion-like propagation of alpha-synuclein, What - if anything - can we learn about neurodegenerative diseases from yeast?, Mitochondrial rejuvenation and replacement as a novel strategy for treatment of age-related neurodegenerative diseases, and more.

Methods in Enzymology, Volume 646, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Methods for Studying RNA condensation/granules in vitro, RNA Dynamics in Intracellular Condensates, Methods for Viscoelastic Characterization of Liquid and Gel Condensates, Incorporating Proteins into Complex Coacervates, Methods for Study of Liquid-Liquid Phase Coexistence in Proximity to Lipid Membranes, Preparation of and Solute Partitioning in Multiphase Coacervates, Reversible photocontrol of DNA coacervation, Enzymatic Control over Coacervation, and much more.

TAM Receptors in Health and Disease, Volume 357 in the International Review of Cell and Molecular Biology, reviews the role of TAM receptors in health and diseases. Chapters in this new release include TAM receptors and its Role in Efferocytosis: Clearance of Dead Cells, TAM family receptors and their ligands: Role in thrombosis, TAM receptors and its ligand mediated activation: Role in Atherosclerosis, Post-translational modifications of the Ligands: Requirement for TAM receptor activation, Immunogenic role of TAM receptors in the cancer microenvironment: Implications in cancer immunotherapy, TAM receptors: A Phosphatidylserine Receptor family and its implications in Viral infections, and much more.

Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level.

Biology of Aminoacyl-tRNA Synthetases, Volume 48 in The Enzymes series, highlights new advances in the field, with this new volume presenting interesting chapters on A narrative about our work on the endless frontier of editing, The puzzling evolution of aminoacyl-tRNA synthetases, Structural basis of the tRNA recognition by aminoacyl-tRNA synthetases, Catalytic strategies of aminoacyl-tRNA synthetases, Trans-editing by aminoacyl-tRNA synthetase-like editing domains, Adaptive and maladaptive mistranslation arising from aminoacyl-tRNA synthetases, Non-canonical inputs and outputs of tRNA aminoacylation, Structure and function of multi-tRNA synthetase complexes, Mitochondrial aminoacyl-tRNA synthetases, Non-canonical functions of human cytoplasmic tyrosyl-, tryptophanyl- and other aminoacyl-tRNA synthetases, and much more.

Extracellular Vesicles, Volume 645 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Genetic labeling of extracellular vesicle exosomes for studying biogenesis and uptake in living mammalian cells, Fluorescent Labeling of Extracellular Vesicles, Isolation of extracellular vesicles from lymph, Transgenic rats for tracking body fluid/tissue-derived extracellular vesicles, Isolation of amniotic extracellular vesicles, Urinary extracellular vesicle isolation, Immunocapture-based ELISA to Characterize and Quantify Extracellular Vesicles in Both Cell Culture Supernatants and Body Fluids, and much more.

Advances in Microbial Physiology, Volume 77, the latest release in this ongoing series, continues the long tradition of topical, important, cutting-edge reviews in microbiology. The updated release contains updates in the field, with comprehensive chapters covering Microbubble Intensification of Bioprocessing, Bacterial cellulose: biosynthesis, production, and applications, Microbial energy management - a product of three broad tradeoffs, and more.

The Microbiome, Volume 176, assembles known facts and provides guidance for their implementation on topics relating to associations between the gut microbiome and personality traits, depression, anxiety, autism, schizophrenia, cognition, dementia and neurodegeneration. Additionally, this volume considers the influence of the maternal microbiome on brain development, with chapters covering Intervention, prevention, and the brain: prebiotics, probiotics, and fecal transplants, The microbiota-gut-brain axis: focus on the fundamental communication pathways, and Microbiome composition and locations.

Membrane Biomechanics, Volume 86, the latest release in the Current Topics in Membranes series, highlights new advances in the field, with this new volume presenting interesting chapters on Lipid bilayers: phase behavior and mechanics, Molecular mechanisms of cell membrane structure modification by omega-3 fatty acids, Mechanical properties of magnetoliposomes, Mechanosensitive ion channels and membrane tension, From cell membrane to the nuclear membrane through modulation of cytoskeleton, Endothelial stiffness in dyslipidemia and aging, Vascular smooth muscle stiffness in aging and vascular disease, Mechanobiology of macrovesicle release and activation, Interplay of membrane cholesterol and substrate on vascular smooth muscle mechanics, and more.

Cosmic Genetic Evolution, Volume 106 in the Advances in Genetics series, highlights new advances in the field, with this new volume presenting interesting chapters on Panspermia, Cometary Panspermia and Origin of Life, The Efficient Lamarckian Spread of Life in the Cosmos, The Sociology of Science and Generality of the DNA/RNA/Protein Paradigm Throughout the Cosmos, The Mutagenic Source and Power of Our Own Evolution, Origin of New Emergent Coronavirus and Candida Fungal Diseases - Terrestrial or Cosmic?, and Future Prospects for Investigation -The Near-Earth Neighborhood and Beyond.

Actin Cytoskeleton in Cancer Progression and Metastasis (Part B), Volume 356 in the International Review of Cell and Molecular Biology series, provides an overview on the roles of the actin cytoskeleton and its key structural regulators, including WASp, Paxillin, Myosin, Testin, L-Plastin and profilin, and in central processes underlying cancer progression and metastasis, such as changes in cell morphology and gene expression, acquisition of migratory and invasive capabilities, and evasion from the immune response. Specific chapters in this release cover Actin dynamics during tumor cell dissemination, Actin cytoskeleton remodeling during cancer cell migration, Cytoskeletal Mechanics Drives Heterogeneity in Epithelial Ovarian Cancer, and much more.

The Enzymes, Volume 47, highlights new advances in the field, with this new volume presenting interesting chapters on The Multipurpose Family of Oxidases, Vanillyl alcohol oxidase, Choline oxidases, Aryl alcohol oxidase, D- and L-amino acid oxidases, Sugar oxidases, Phenolic Compounds hydroxylases, Baeyer-Villiger Monooxygenases, Flavin-dependent halogenases, Flavin-dependent dehalogenases, Styrene Monooxygenases, Bacterial luciferases, Cellobiose Dehydrogenases, Prenylated flavoenzymes, Ene-reductases, Flavoenzymes in Biocatalysis.

Enzymes - Mechanisms, Dynamics and Inhibition, Volume 122, the latest release in the Advances in Protein Chemistry and Structural Biology series, highlights new advances in the field, with this new volume presenting new and interesting chapters on the topics. Each chapter is written by an international board of authors.

Methods in Enzymology, Volume 644, the latest release in this ongoing serial, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Site-directed recombination (SDR) in vivo: a fast and reliable tool to unveil beneficial epistasis, Creation and application of amine oxidase with expanded substrate specificities from porcine kidney D-amino acid oxidase, Methods to assess correlation networks for engineering transketolase, Exploration of Enzyme Diversity by Integrating Bioinformatics with Microfluidics, Engineering lytic polysaccharide monooxygenases (LPMOs), Emulsion-based directed evolution of enzymes in yeast, and much more.

Plant Cell Biology, volume 160 in "Methods in Cell Biology", includes chapters on modern experimental procedures and applications developed for research in the broad area of plant cell biology. Topics covered in this volume include techniques for imaging and analyzing membrane dynamics and movement across membranes; cell wall composition, structure and mechanics; cytoskeleton dynamics and organization; cell development; ion channel physiology; cell mechanics; and methods related to quantifying cell morphogenesis.

What Arieh Warshel and fellow 2013 Nobel laureates Michael Levitt and Martin Karplus achieved - beginning in the late 1960s and early 1970s when computers were still very primitive - was the creation of methods and programs that describe the action of biological molecules by 'multiscale models'. In this book, Warshel describes this fascinating, half-century journey to the apex of science.From Kibbutz Fishponds to The Nobel Prize is as much an autobiography as an advocacy for the emerging field of computational science. We follow Warshel through pivotal moments of his life, from his formative years in war-torn Israel in an idealistic kibbutz that did not encourage academic education; to his time in the army and his move to the Technion where he started in his obsession of understanding the catalytic power of enzymes; to his eventual scientific career which took him to the Weizmann Institute, Harvard University, Medical Research Council, and finally University of Southern California. We read about his unique contributions to the elucidation of the molecular basis of biological functions, which are combined with instructive stories about his persistence in advancing ideas that contradict the current dogma, and the nature of his scientific struggle for recognition, both personal and for the field to which he devoted his life. This is, in so many ways, more than just a memoir: it is a profoundly inspirational tale of one man's odyssey from a kibbutz that did not allow him to go to a university to the pinnacle of the scientific world, highlighting that the correct mixture of persistence, talent and luck can lead to a Nobel Prize.