Integrated Methods in Protein Biochemistry: Part A, Volume 677, the latest release in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on topics such as DNA and protein engineering to create protein bioswitches with new functions, Interaction and cross-talk of prelamin A with integral membrane zinc metalloproteases, An experimental protocol to study lipid transfer proteins, Synthesis of small heat shock proteins, Druggable p-p interacting sites for Co-chaperone DNAJA1 and its partner proteins, An experimental protocol for glycoconjugate analysis, Methods for proximity-based biotinylation combined with Mass Spectrometry, and more. Additional chapters cover Synthetic antibody fragments as conformational sensors of protein activation and trafficking, Expression, purification, functional analysis and crystallization of Rag GTPase, Purification of bacterial transcription elongation complexes by photoreversible immobilization, Inhibition of c-Myc-MAX heterodimerization, Fluorogenic RNA aptamers to probe transcription by multi-subunit RNA polymerases, and much more.

Sustainable Plant Nutrition: Molecular Interventions and Advancements for Crop Improvement explores the significant opportunities for sustainable, eco-friendly approaches in plant nutrition and agricultural crop production. The book highlights the various prospects involved in optimizing plant nutrient uptake agriculture and includes chapters representing diverse areas dealing with biotechnology, nanotechnology, molecular biology, proteomics, genomics and metabolomics. This book is an ideal resource for those seeking to ensure a sustainable plant production future. While plants have evolved a set of elaborate mechanisms to cope with nutrient limitations, the traditional supplementation by the application of fertilizers to plant productivity may then lead to overfertilization which can actually reduce plant growth and have adverse effects on the environment. To tackle these issues, a detailed understanding of the responses of plants to nutrients and nutrient deficiency at the physiological, metabolic, transcriptome and epigenetic level is essential.

Carotenoids: Carotenoid and Apocarotenoid Biosynthesis, Metabolic Engineering and Synthetic Biology, Volume 671, the latest release in the Methods of Enzymology series highlights new advances in the field with chapters on Metabolomics-based analysis of carotenoids and related metabolites in various species via quantitative trait loci and genome wide association mapping approaches, Using bacteria for functional analysis of genes encoding carotenoid biosynthetic enzymes, Rice Callus as a High Throughput Platform for Synthetic Biology and Metabolic Engineering of Carotenoids, Transient expression in Nicotiana benthamiana: A simple platform to investigate genes encoding carotenoid biosynthesis enzymes from diverse algal lineages, and much more. Additional chapters in this new release cover Protein-protein interaction techniques to investigate post-translational regulation of carotenogenesis, The isolation of sub-chromoplast structures from tomato and capsicum fruit, Carrot protoplasts as a suitable method for protein subcellular localization, High throughput production and characterization of carotenoid enzymes for structural and functional studies, Production and structural characterization of the cytochrome P450 enzymes in carotene ring hydroxylation, and more.

Adoptive Cell Transfer, Volume 370 in the International Review of Cell and Molecular Biology series highlights advances in the field, with this new volume presenting interesting chapters written by an international board of authors who expound on topics such as the Impact of tumor microenvironment on Adoptive Cell Transfer activity, Dendritic Cell Transfer, CAR-T Cell dysfunction and exhaustion, NK Cell-based cancer immunotherapy, Enabling CAR-T cells for solid tumors: rage against the suppressive tumor microenvironment, Improving Adoptive T-Cell therapy with cytokines administration, and What will (and should) be improved in Immunotherapy with CAR?

Protein Interaction Networks, Volume 131 in the Advances in Protein Chemistry and Structural Biology series, highlights new advances in the field, with this new volume presenting interesting chapters written by an international board of authors.

New Antibody Formats, Volume 369 in the International Review of Cell and Molecular Biology serial, highlights new advances in the field with this new volume presenting interesting chapters on a variety of important topics, including Current innovative engineered antibodies, Advances in bispecific antibodies, Applications of trimerbodies for cancer immunotherapy, Multispecific mAb-Anticalin formats, New antibody formats to treat Lung Cancer, Immunocytokines: from bench to bedside, and Single domain antibodies in cancer.

Coenzyme B12 Enzymes, Part B, Volume 169 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on Structural characterization of cobalamin-dependent radical SAM methylases, Purification and characterization of sequential cobalamin-dependent radical SAM methylases ThnK and TokK in -lactam antibiotic biosynthesis, Characterization of the cobalamin-dependent radical S-adenosyl-L-methionine enzyme C-methyltransferase Fom3 in fosfomycin biosynthesis, Studies of OxsB and GenK, two B12-dependent radical SAM enzymes involved in natural product biosynthesis, Purification and structural elucidation of the cobalamin-dependent radical SAM enzyme OxsB, and more. Other chapters discuss Methods for studying the mechanisms of B12 enzymes, Computational investigations of B12 dependent enzymatic reactions, Using kinetic isotope effects to probe the mechanisms of adenosylcobalamin-dependent enzymes, Steady-state and pre-steady state kinetic analysis of ornithine 4,5-aminomutase, and more.

Cyanobacteria are ancient, primordial oxygenic phototrophs, and probably the progenitor of oxygen-evolving photosynthesis. They are a prolific source of natural products and metabolites and vitally important for environmental biology and biotechnology. Cyanobacterial Physiology presents foundational knowledge alongside the most recent advances in cyanobacterial biology. The title examines the challenges of industrial application through an understanding of the basic molecular machinery of cyanobacteria. Sixteen chapters are organized into three sections. The first part covers basic cyanobacterial biology, emphasizing environmental biology such as photosynthesis, nitrogen fixation, circadian rhythm, and programmed cell death. The second part includes the chapters that discuss cyanobacterial extremophiles, adaptations, secondary metabolites, osmoprotectants, and toxins. The third part covers aspects of cyanobacterial application that are based on environmental biology. Leading scientists contribute chapters on cyanobacteria. Cyanobacterial Physiology presents a comprehensive and vibrant solution for researchers and engineers in biotechnology interested in cyanobacteria and their applications. Topics include the cyanobacterial cell and fundamental physiological processes; the biotechnological potential of cyanobacteria with their versatile metabolism; and advanced applications of cyanobacterial products. At each stage the book is informed by basic and applied research.

One, No One, One Hundred Thousand - The Multifaceted Role of Macrophages in Health and Disease - Part B, Volume 369 provides in-depth reviews on the last progresses on the role of macrophages in health and diseases, with a special focus on the role of macrophages during development. New chapters cover The role of salivary gland macrophages in infection, disease and repair, Reprogramming or replacing brain macrophages to treat neurodegenerative disease, Targeting macrophages for cancer immunotherapy, Heart Macrophages at steady-state and disease: simple bystanders or active players?, Macrophages in the liver, and Developmental programming of macrophages.

Covid-19: Biomedical Perspectives, Volume 50 in the Methods in Microbiology series highlights new advances in the field, with this new volume presenting interesting chapters written by an international board of authors. Individual chapters in this new release include Sensitive methods for detection of SARS-CoV-2 RNA, Treatment of COVID-19 using Chinese herbal medicine, Understanding how SARS-CoV-2 is evolving and its impact on COVID-19 animal models and vaccine evaluation, Methods in machine learning to identify COVID-19 literature, COVID-19 seasonal behavior and the mutational landscape of the SARS-CoV-2 virus, CRISPR use in Diagnosis and Therapy for COVID-19, and much more.

Large Animals as Models for Human Diseases, Volume 189 in the Progress in Molecular Biology and Translational Science series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of important topics, including Sheep as a model for neuroendocrinology research, Relevance of dog as a model for urologic diseases, Relevance of lactocrine hypothesis to human maternal programming of development, Comparative aspects of embryo implantation, Pig as an animal model for obesity, Canine models of cancer, Bovine model for human ovarian diseases, Mutations in G protein-coupled receptors in large animals: Modeling human diseases, and more.

B12 Enzyme, Volume 667 in the Methods in Enzymology serial, highlights new advances in the field with this new volume presenting timely, interesting chapters, including Part I: B12 Biosynthesis and B12 Chaperones, Probing the preference and regioselectivity of lower ligand activation and its role in cobamide diversity, Biosynthesis of corrinoids, The human B12 trafficking chaperones: CblC, CblD and ATR, A method for the efficient adenosylation of corrinoids, Production, purification and liposome reconstitution of cobamide synthase, Extraction and Purification of Cobamides from Bacterial Cultures, Part II: The many faces of B12-dependent enzyme reactions, Methylcobalamin-dependent methionine synthase, and much more.

Carotenoids: Carotenoid and Apocarotenoid Analysis, Volume 670, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this new volume covering Getting to know carotenoids, Laser capture of tissues for micro-scale carotenoid analyses, Metabolic engineering of carotenoids: procedures for metabolomic characterization, LC-MS analysis of intracellular metabolites for precursors to the carotenoid pathway, Use of E. coli to produce carotenoid standards, HPLC analysis of carotenoids from Bacteria, Purification and development of standards for carotenoid quantification in plant tissues, and much more. Additional sections in this release cover Ultra-High Performance Liquid Chromatography-Mass Spectrometry Analysis of Plant Apocarotenoids, Detection and analysis of novel and known volatile plant apocarotenoids, Carotenoid extraction, detection, and analysis in citrus, Strategies For The Separation And Tentative Identification Of Geometrical (Cis/Trans, Z/E) Isomers Of Carotenoids, Use of stable isotopes to study bioconversion and bioefficacy of pro-vitamin A carotenoids, Carotenoid extraction and analysis of blood plasma/serum, and more.

Pseudokinases, Volume 667, the latest release in the Methods in Enzymology serial, highlights new advances in the field with this new volume presenting interesting chapters, including the Production and Purification of the PEAK pseudokinases for structural and functional studies, Structural biology and biophysical characterization of Tribbles pseudokinases, Detecting endogenous TRIB protein expression and its downstream signaling, Analysis of human Tribbles 2 pseudokinase, Expression, purification and examination of ligand-binding to IRAK pseudokinases, Characterization of pseudokinase ILK-mediated actin assembly, Biochemical examination of Titin pseudokinase, Approaches to study pseudokinase conformations, CRISPR editing cell lines for reconstitution studies of pseudokinase function, and much more.

Methods in Cancer Stem Cell Biology: Part A, Volume 170 in the Methods in Cell Biology series highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Orthotopic brain tumor models derived from glioblastoma stem-like cells, RNA sequencing in hematopoietic stem cells, Generation of inducible pluripotent stem cells from human dermal fibroblasts, In vitro preparation of dental pulp stem cell grafts combined with biocompatible scaffolds for tissue engineering, Gene expression knockdown in chronic myeloid leukemia stem cells, Identification and isolation of slow-cycling GSCs, Assessment of CD133, EpCAM, and much more.

Advances in Biomolecular EPR, Volume 666 in the Methods of Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics including Magnetic Resonance Characterization of Physiologically Important Metal Ion Binding Sites in the Prion and Related Proteins, The catalytic role of metal-radical/protein-based radicals in heme enzymes, Rigid Cu2+-based spin labels for the study of higher-order DNA G-quadruplex structures, Orthogonal spin labeling and membrane proteins: increasing the information content and going towards in cell applications, Spectroscopic investigation of mono- and di-Mn-containing centers in biochemistry with an emphasis on application of paramagnetic resonance techniques, and more. Additional chapters cover In Vivo pO2 Imaging of Tumors: Oxymetry with Very Low-Frequency Electron Paramagnetic Resonance, an Update, EPR contributions to understanding molybdenum-containing enzymes, EPR spectroscopy of Type I reaction centers, Characterization of a substrate-derived radical in the NosN reaction during the biosynthesis of nosiheptide, and much more.

Protein Design and Structure, Volume 130 in the Advances in Protein Chemistry and Structural Biology series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.

Advances in Microbial Physiology, Volume 150 in this important serial, highlights new advances in the field with this new volume including content by an international board of authors. Chapters in this new release include Organization of respiratory chains in the bacterial cell, Anaerobic methane oxidizing archaea, Dawn of the DedA: the structure and function of the DedA family of integral membrane proteins associated with bacterial viability and antimicrobial resistance, Nickel, an essential virulence determinant of Helicobacter pylori: trafficking pathways and their targeting by bismuth, Dissimilatory sulfur compounds oxidation in thermophilic and chemolithoautotrophic bacteria belonging to the Aquificales order, and much more.

One, No one, One Hundred Thousand - The Multifaceted Role of Macrophages in Health and Disease - Part A, provides in-depth reviews on the last progresses on the role of macrophages in health and diseases with a special focus on the role of macrophages in SARS-COV2, breast cancer, liver and salivary glands.

Chemical Microbiology, Part B, Volume 665, the latest release in the Methods of Enzymology series, highlights new advances in the field, including comprehensive chapters on the Application of Antibiotic-derived Fluorescent Probes to Bacterial Studies, Metabolomic approaches for enzyme function and pathway discovery in bacteria, Adding a diazo-transfer reagent to culture to generate secondary metabolite probes for click chemistry, Customized Peptidoglycan Surfaces to Investigate Innate Immune Recognition via Surface Plasmon Resonance, Development and application of highly sensitive labeling reagents for amino acids, Bacterial Cell Wall Modification with a Glycolipid Substrate, and much more.

Selenoprotein Structure and Function, Volume 662 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters written by an international board of authors. Chapters in this new release include Identification of Selenoprotein O substrates using a biotinylated ATP analog, Selenium-encoded isotopic signature targeted profiling, Designing tRNASec variants for efficient selenocysteine incorporation using Sec-specific reporters, Preparation of selenoprotein S by chemical ligation, Examining xCT-mediated selenium uptake and selenoprotein production capacity in cells, SecMS analysis of selenoprotein with selenocysteine insertion sequence and beyond, Selenocysteine substitutions in thiyl radical enzymes, and much more. Additional chapters cover Recombinant selenoprotein expression in E. coli based upon the redefinition of a UAG codon in an RF1-depleted host strain, Metabolic labeling with radioactive selenium in zebrafish, Low pH isoTOP-ABPP to identify selenocysteines, Expression of selenoproteins via genetic code expansion in mammalian cells, Alpha-methyl selenocysteine as a tool for the study of selenoproteins, Selective selenol fluorescent probes: design, synthesis, structural determinants, and biological applications, and much more.

Micro/Nanofluidics and Lab-on-Chip Based Emerging Technologies for Biomedical and Translational Research Applications - Part B, Volume 187 represents the collation of chapters written by eminent scientists worldwide. Chapters in this new release include Design and fabrication of microfluidics devices for molecular biology applications, Micro/Nanofluidics devices for drug delivery, From organ-on-chip to body-on-chip: the next generation of microfluidics platforms for in vitro drug toxicity testing, Micro/Nanofluidics for high throughput drug screening, Design, fabrication and assembly of lab-on-a-chip and its uses, Advances in microfluidic 3D cell culture for pre-clinical drug development, Tissue and organ culture on lab-on-a chip for biomedical applications, and much more.

Biomolecular Interactions: Part A, Volume 169, the latest release in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including Emerging Mechanisms of Targeted Protein Degradation by Molecular Glues, Design and use of programmable DNA Hydrogels, Oligomerization of membrane receptors: Approaches to measure in live cells, Interactions of alpha-synuclein with biomolecules, Gel-electrophoresis based method for biomolecular interaction, Recombinant centrosome expression in bacterial system, Reconstituting CCL5-CCR5 complex for structural and mechanistic analysis, Protein engineering and design in ion channel receptors, and much more.