A method to fabricate porous silicon membranes as thin as 1
micrometer for analysis of transmembrane proteins is detailed. This
comprehensive presentation includes: 1) The detailed process to
fabricate porous silicon membranes that are supported from the
sides. The porous silicon membrane acts as a sieve that separates
two compartments. 2) A procedure to deposit a viable and robust
synthetic lipid bilayer membrane on the porous silicon, and
incorporate transmembrane proteins into it. This procedure includes
answers to what technique to use for lipid bilayer deposition, and
which lipids to choose for incorporating proteins. This lipid
bilayer membrane acts as a plasma membrane in living cells. 3) A
comprehensive analysis, characterization, and assessment of the
complete process and each individual step is performed using
electrochemical impedance spectroscopy (EIS), atomic force
microscopy (AFM), and scanning electron microscopy (SEM). The rigid
porous wall and the lipid membrane with fused functional
transmembrane proteins result in a device that resembles the
cellular wall of the E. Coli bacteria. Numerous membrane proteins
may be analyzed using this device.
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