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Doubled Haploid Production in Crop Plants - A Manual (Hardcover, 2003 ed.)
Loot Price: R5,978
Discovery Miles 59 780
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Doubled Haploid Production in Crop Plants - A Manual (Hardcover, 2003 ed.)
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This is the first manual presenting a set of protocols for
production of doubled haploids (DH) in 22 major crop plant species
including 4 tree species. It contains various protocols and
approaches of DH production proven for different germplasm of the
same species. The protocols describe in detail all steps of DH
production - from donor plants growth conditions, through in vitro
procedures, media composition and preparation, to regeneration of
haploid plants and chromosome doubling methods. The users of this
manual will be able to choose among microspore versus anther
cultures, wide hybridisation or gynogenesis, the most suitable
method for production of DH in particular laboratory conditions for
their plant material. Numerous colour illustrations should help in
this decision. The user will also find information on organization
of a doubled haploid laboratory, basic DH media and on associated
simple cytogenetic methods for ploidy level analysis. The practical
protocols are supplemented with the list of published DH protocols
for other crop plants and separate chapters dealing with major
applications of DH in breeding, mutant production, transgenesis,
and genetic mapping and genomics.
The book was prepared by the Plant Breeding and Genetics Section of
the Joint FAO/IAEA Division of Nuclear Techniques in Food and
Agriculture in close co-operation with the EU program COST 851' on
Gametic cells and molecular breeding for crop improvement'.
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