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Molecular Methods in Developmental Biology - Xenopus and Zebrafish (Hardcover, 1999 ed.)
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Molecular Methods in Developmental Biology - Xenopus and Zebrafish (Hardcover, 1999 ed.)
Series: Methods in Molecular Biology, 127
Expected to ship within 10 - 15 working days
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The process whereby a single cell, the fertilized egg, develops
into an adult has fascinated for centuries. Great progress in
understanding that process, h- ever, has been made in the last two
decades, when the techniques of molecular biology have become
available to developmental biologists. By applying these
techniques, the exact nature of many of the interactions
responsible for forming the body pattern are now being revealed in
detail. Such studies are a large, and it seems ever-expanding, part
of most life-science groups. It is at newcomers to this field that
this book is primarily aimed. A number of different plants and
animals serve as common model org- isms for developmental studies.
In Molecular Methods in Developmental Bi- ogy: Xenopus and
Zebrafish, a range of the molecular methods applicable to two of
these organisms are described, these are the South African clawed
frog, Xenopus laevis, and the zebrafish, Brachydanio rerio. The
embryos of both of these species develop rapidly and externally,
making them particularly suited to investigations of early
vertebrate development. However, both Xenopus and zebrafish have
their own advantages and disadvantages. Xenopus have large, robust
embryos that can be manipulated surgically with ease, but their
pseudotetraploidy and long generation time make them unsuitable
candidates for genetics. This disadvantage may soon be overcome by
using the diploid Xenopus tropicalis, and early experiments are
already underway. The transp- ent embryos of zebrafish render them
well-suited for in situ hybridization and immunohistochemistry, and
good for observing mutations in genetic screens.
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