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Books > Medicine > Pre-clinical medicine: basic sciences > Anatomy
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Skeletal Muscle Regeneration in the Mouse - Methods and Protocols (Hardcover, 1st ed. 2016)
Loot Price: R5,771
Discovery Miles 57 710
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Skeletal Muscle Regeneration in the Mouse - Methods and Protocols (Hardcover, 1st ed. 2016)
Series: Methods in Molecular Biology, 1460
Expected to ship within 10 - 15 working days
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This volume focuses on the cell biology and physiology of skeletal
muscle regeneration. This Book is a collection of classic and
cutting edge protocols optimized for mice, but in most cases
adaptable to rat or other mammalian models, that will allow an
investigator to develop and implement a research study on skeletal
muscle regeneration. Chapters address the three major areas of
study: provoking regeneration by inducing damage to muscle,
analyzing the progenitor cells of skeletal muscle, and quantifying
overall muscle function. Subjects discussed include: inducing
skeletal muscle injury by eccentric contraction; volumetric muscle
loss; single myofiber isolation and culture; satellite cell
transplantation; muscle clearing for whole mount immunostaining;
luciferase tracking of muscle stem cells; mitochondrial and
mitophagy flux analysis; in vivo assessment of muscle
contractility; force measurements on single isolated myofibers; and
analysis of aerobic respiration in intact skeletal muscle tissue by
microplate respirometry. Written in the highly successful Methods
in Molecular Biology series format, chapters include introductions
to each respective topic, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls. Cutting
edge and practical, Skeletal Muscle Regeneration in the Mouse:
Methods and Protocols is an essential laboratory reference for
research in skeletal muscle growth, damage, repair, degeneration,
and regenerative therapy in the mouse model system.
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