Extending the range of enzymatic catalysis by using non-aqueous
media has now developed into a powerful approach in biochemistry
and biotechnology. One peculiar feature which distinguishes it from
the conventional enzymology (carried out in aqueous buffers) is
that the awareness of different parameters that control and
influence the behaviour of enzymes in such environments has emerged
rather slowly. Science is about being able to repeat what somebody
else has done. Absence of knowledge about such well-defined
parameters/fac tors has sometimes made some workers rather cautious
and diffident about using this approach in their laboratories. But
for this, non-aqueous enzymol ogy would be more widely practised.
It is these thoughts that made me feel that the availability of
some well-defined protocols for various applications invol ving
enzymes in non-aqueous environments would further catalyze the
growth of this area. Hence this book, in which each chapter has
some protocols in a specific area. The protocols are preceded by
brief background material. The early chapters, which are of general
importance, concern control of water ac tivity and stabilization
via immobilization. Some subsequent chapters provide the protocols
for transformations involving lipids and carbohydrates, peptide
synthesis, and preparation of chiral compounds. The
disproportionate focus on lipases is not a coincidence; this class
of enzymes has been used more often than others in non-aqueous
enzymology.
General
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