Although previously thought to be merely passive structural
components, membrane lipids have recently been found to be actively
involved in cellular transport and signal transduction processes.
Clear protocols for the study of membrane lipid properties,
cellular transport or signal transduction are presented in this
manual. Following a short introduction to membrane lipids,
techniques for the isolation and extraction of membrane fractions,
the analysis of the lipid composition, lipid turnover, and the
involvement in signal transduction as well as the preparation of
liposomes are described.
General
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