Present investigation developed three pathways for in vitro
regeneration and mass multiplication of Acacia mangium. The nodal
segments from 8-10 years old phenotypically superior plus tree were
used as explants for axillary bud culture. MS medium supplemented
with BAP and Kinetin in combination was the best for culture
establishment and shoot proliferation from the axillary buds.
Microshoots were rooted in MS medium (half strength) supplemented
with IBA and acclimatization of the rooted shoots was obtained in
plastic pots containing soilrite. Sixty per cent of cotyledon
explants developed direct adventitious buds. Callus was induced
from the cotyledon and hypocotyl explants on MS medium fortified
with NAA and BAP. Genetic fidelity of micropropagated plants was
examined by RAPD analysis, at every subculture. No genetic
variation was detected among plantlets, hence proving genetically
stable regeneration method. This protocol can be very useful for
for genetic engineering and mass production of quality planting
material of Mangium.
General
| Imprint: |
Lap Lambert Academic Publishing
|
| Country of origin: |
Germany |
| Release date: |
February 2012 |
| First published: |
February 2012 |
| Authors: |
Rajveer Singh Chauhan
• Suman Kumar Jha
• Sanjay Jha
|
| Dimensions: |
229 x 152 x 8mm (L x W x T) |
| Format: |
Paperback - Trade
|
| Pages: |
128 |
| ISBN-13: |
978-3-8473-7799-3 |
| Categories: |
Books
|
| LSN: |
3-8473-7799-X |
| Barcode: |
9783847377993 |
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