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Experimental Approaches For The Investigation Of Innate Immunity: The Human Innate Immunity Handbook (Hardcover)
Loot Price: R2,247
Discovery Miles 22 470
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Experimental Approaches For The Investigation Of Innate Immunity: The Human Innate Immunity Handbook (Hardcover)
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The recent explosion of information in innate immune pathways for
recognition, effect or responses, and genetic regulation has given
impetus to investigations into analogous pathways in the human
immune response, which in turn has produced attendant insights into
both normal physiology and immunopathology. This volume presents a
compendium of methods and protocols for the investigation of human
innate immunity with application to the study of normal immune
function, immunosenescence, autoimmunity and infectious diseases.
Among the topics covered are quantitative flow cytometry for
Toll-like receptor expression and function; multidimensional single
cell mass cytometry (CyTOF) in complex immune interactions and
tumor immunity; imaging techniques such as Imagestream high
resolution microscopy coupled to flow cytometry, immune cell
infiltration of organotypic, biomimetic organs; high-throughput
single cell secretion profiling; multiplexed transcriptomic
profiling; microsatellite and microRNA methodologies, RNA
interference; and the latest bioinformatics and biostatistical
methodologies, including in-depth statistical modeling, genetic
mapping, and systems approaches.
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