The detection and/or isolation and identification of pathogenic
microorganisms is critical for the laboratory diagnosis of
infectious diseases. With growth-dependant methods providing
reliable means for identifying pathogens, traditional culturing
continues to play an integral role in the detection and
characterization of known and "new" microbial pathogens.
Microbiologists, therefore, rely on a variety of media for the
detection, isolation, characterization, and identification of
primary and opportunistic microbial pathogens.
The Handbook of Media for Clinical and Public Health
Microbiology provides a compilation of the formulations, methods of
preparation, and applications for media used in clinical and public
health microbiology laboratories. It is a significant update to the
"Handbook of Media for Clinical Microbiology," expanding the
coverage to media used for public health epidemiological
investigations of disease outbreaks and including media used for
the detection of pathogens in foods and environmental samples.
Comprising both classic and modern media, the handbook describes
almost 1,800 types of media, listed alphabetically, including new
media for the cultivation of emerging bacteria, fungi, and viruses
that are causing major medical problems around the world. Examples
of emerging pathogens are extended-spectrum beta-lactamase
(ESBL)-producing bacteria, "Escherichia coli "O157: H7,
methicillin-resistant "Staphylococcus aureus "(MRSA),
vancomycin-resistant enterococci (VRE), and carbapenem-resistant
"Enterobacteriaceae "(CRE). Many of the new media contain
chromogenic or fluorogenic substrates that permit rapid detection
of specific pathogens.
The handbook s format allows easy reference to information needed
to prepare media for cultivating clinically relevant
microorganisms. It also contains descriptions of expected results
for organisms that are important for the examination of foods,
water, and other specimens of public health significance as well as
clinical specimens.
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