A compendium of quality Caprine Mycoplasma vaccine production is
presented with respect to its stability, safety and sterility
studies and in-vivo clinical trial of plain formalin inactivated
(FIV) and two adjuvanted vaccines (AAV & OEV)belong to
Mycoplasma mycoides subspecies capri (PG3) also known as Mycoplasma
capricolum sub spp capricolum in goats. The growth was obtained in
PPLO broth containing yeast extract (1%), horse serum (20%) and
thallium acetate. Each dose of 2.5ml vaccine contains not less than
0.10 mg of protein. All the vaccines were found stable upto 6
months under refrigeration temperature.Indirect haemagglutination
test (IHA) and complement fixation tests (CFT) were applied to
determine humoral immune response in goats. Overall vaccine results
showed that OEV had produced higher CFT antibody response. Whereas,
AAV alone and as booster showed non significant variation in the
CFT and IHA antibody response. This is the first reported effort to
develop adjuvanted vaccines against Mycoplasma mycoides subspecies
capri (PG3) to develop immunity in goats. Moreover, OEV and AAV
proved helpful in providing solid immunity which lasted for more
than a year.
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