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Quantitative Proteomics by Mass Spectrometry (Paperback, Softcover reprint of hardcover 1st ed. 2007)
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Quantitative Proteomics by Mass Spectrometry (Paperback, Softcover reprint of hardcover 1st ed. 2007)
Series: Methods in Molecular Biology, 359
Expected to ship within 10 - 15 working days
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Quantitative Proteomics by Mass Spectrometry, from the Methods in
Molecular Biology series, is a compendium of cutting-edge protocols
for quantitative proteomics, and presents the most significant
methods used in the field today. The focus on mass spectrometry
(MS) is integral, as MS has, and will continue to be, an essential
tool in proteomics for studying complex biological systems and
human diseases. This volume, written and compiled by leading
quantitative proteomic experts, is an indispensable resource in the
search for novel biomarkers.
Quantitative Proteomics by Mass Spectrometry presents several
innovative MS quantitative procedures, including a variety of
methods for introducing isotopic labels and quantifying
post-translational modifications. Some of these methods include
growing an organism in isotope-enriched media, performing trypsin
proteolysis in the presence of 18O-water, reacting protein samples
with isotopically labeled reagents, quantifying relative amount of
proteins without the use of any isotopic labels. Attention is also
given to state-of-the-art techniques for the characterization of
the phosphoproteome and tandem MS for detection of inborn errors of
metabolism. Specifically, the procedure for determinations of
enzymatic activity could be used for large-scale screening of
newborns. The protocols in this volume expand both the breadth and
depth of readily available methods for quantitative proteomic
researchers using MS.
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