Molecular markers are useful for precise assessment of diversity
and phylogenetic relationships among different species and related
genera at the DNA level without some of the over simplifying
assumptions associated with calculating genetic diversity based on
pedigree history. The objective of present study was to have a
basic understanding of the genetic diversity that exists in the
germplasm available for breeding, which is fundamental for the
success of any breeding program. For this study 40 sugarcane
accessions were collected from the germplasm source. The accessions
consist of advance lines, approved varieties and wild species (S.
barberi). Random Amplified Polymorphic DNA (RAPD) and
Micro-satellite (SSR) analysis was performed using 80 primers. Use
of SSR and RAPD primers together offers the potential of more cost
effective data acquisition than is the case with other
technologies. These primers sequence vary in different varieties of
sugarcane, and this variability may be used to develop molecular
markers for mapping sugarcane genes and traits, these sequences
being the part of sugarcane genome predicted to be most immediately
useful to plant breeder and geneticists.
General
Imprint: |
Lap Lambert Academic Publishing
|
Country of origin: |
Germany |
Release date: |
2012 |
First published: |
2012 |
Authors: |
Sehar Nawaz
• Smi Ullah
• Aqeel Afzal Khan
|
Dimensions: |
229 x 152 x 6mm (L x W x T) |
Format: |
Paperback - Trade
|
Pages: |
96 |
ISBN-13: |
978-3-8473-7934-8 |
Categories: |
Books
|
LSN: |
3-8473-7934-8 |
Barcode: |
9783847379348 |
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