MicroRNA Profiling - Methods and Protocols (Hardcover, 1st ed. 2017)

This volume includes comprehensive descriptions of miRNA biogenesis and their role in the development and progression of various human diseases. The first few chapters of MicroRNA Profiling: Methods and Protocols discuss the effects of over-expressing and repressing of a target miRNA and their effects on cell viability and proliferation. The next few chapters explore the protocols for total RNA isolation from cells and cell-derived product including formalin fixed paraffin embedded tissue and plant tissue. The last few chapters discuss isolation and characterization of exosomes from medium conditioned by cell lines, serum, and plasma specimens. This book also includes discussions of several software tools, such as miRandola, PicTar, DIANA, and miRWalk. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, MicroRNA Profiling: Methods and Protocols is a valuable resource for anyone interested in the field of Micro RNAs.

General

Imprint:	HumanaPress
Country of origin:	United States
Series:	Methods in Molecular Biology, 1509
Release date:	November 2016
First published:	2017
Editors:	Sweta Rani
Dimensions:	254 x 178 x 17mm (L x W x T)
Format:	Hardcover
Pages:	246
Edition:	1st ed. 2017
ISBN-13:	978-1-4939-6522-9
Categories:	Books > Medicine > Pre-clinical medicine: basic sciences > Medical genetics Books > Science & Mathematics > Biology, life sciences > Molecular biology Books > Medicine > Pre-clinical medicine: basic sciences > Anatomy > General Books > Science & Mathematics > Biology, life sciences > Cellular biology > General
LSN:	1-4939-6522-0
Barcode:	9781493965229

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