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Hydrocarbon and Lipid Microbiology Protocols - Single-Cell and Single-Molecule Methods (Hardcover, 1st ed. 2016)
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Hydrocarbon and Lipid Microbiology Protocols - Single-Cell and Single-Molecule Methods (Hardcover, 1st ed. 2016)
Series: Springer Protocols Handbooks
Expected to ship within 12 - 17 working days
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This Volume presents relevant single-cell and single-molecule
approaches in the study of microbes producing and utilizing
hydrocarbons and lipids. While generically applicable for all
microorganisms, the approaches described are, wherever possible,
adapted to the field of study of hydrocarbon and lipid
microbiology. The methods include basic procedures for isolating
single cells by means of microfluidics and flow cytometry, and
their cultivation in arrays as pure clones; for isolating,
amplifying and sequencing single-cell genomes and transcriptomes;
and for analysing single-cell metabolomes by means of Raman
spectroscopy. Single-molecule approaches include the use of
protein:fluorescent dye fusions for protein localization and
methods for the production of cell division protostructures and
lipid monolayers. Methods for the functional analysis of single
cells include detection of metabolically active
(protein-synthesizing) cells in environmental samples by
bioorthogonal non-canonical amino acid tagging, Raman spectroscopy
combined with stable isotope labelling and fluorescent in situ
hybridisation, and visualization of single cells participating in
gene transfer activity. Lastly, protocols are presented for
single-cell biotechnological applications, including biofuel
production. Hydrocarbon and Lipid Microbiology ProtocolsThere are
tens of thousands of structurally different hydrocarbons,
hydrocarbon derivatives and lipids, and a wide array of these
molecules are required for cells to function. The global
hydrocarbon cycle, which is largely driven by microorganisms, has a
major impact on our environment and climate. Microbes are
responsible for cleaning up the environmental pollution caused by
the exploitation of hydrocarbon reservoirs and will also be pivotal
in reducing our reliance on fossil fuels by providing biofuels,
plastics and industrial chemicals. Gaining an understanding of the
relevant functions of the wide range of microbes that produce,
consume and modify hydrocarbons and related compounds will be key
to responding to these challenges. This comprehensive collection of
current and emerging protocols will facilitate acquisition of this
understanding and exploitation of useful activities of such
microbes.
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