This protocol book provides detailed procedures for the isolation
of cyanobacteria, extraction, quantification, and detection of
cyanotoxins. It illustrates the sampling and processing of
toxin-producing cyanobacteria in water and aquatic animal samples,
detection of cyanotoxins from Anabaena, Anabaenopsis,
Cylindrospermopsis, Microsystis, Microcystis, Nodularia, Nostoc,
Schizotrix, Lyngbya, Raphidiopsis, Oscillatoria, Planktothrix in
aquatic resources. It also covers toxicity analysis by various
bioassay protocols, and in vitro and insilico analysis methods. The
book also reviews the methods for cyanotoxin extraction, detection,
and quantification by various tools including LC-MS/MS, HPLC, NMR,
PCR, and HESI-MS/MS. A separate section is dedicated to the
advanced methods in Cyanotoxin analysis including the Molecular
Imprinting Method (MIM), Cellular signaling biosensor,
Electrochemical sensor, Nanosensors, and screening of Polyketide
synthase gene. The analysis of various toxin-producing genes like
sxtA and mcy is also accounted for in the proposed book. In a
nutshell, the book gives comprehensive procedures about the basics
and preliminary processes that are involved in sample collection to
advanced methods incorporated into the well-explored and unexplored
Cyanobacterial toxin. Consequently, the proposed manual is useful
for both beginners and advanced researchers, including postgraduate
students, academicians, researchers, and scientists in the field of
Cyanobacterial research. ​
General
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