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Books > Science & Mathematics > Biology, life sciences > Molecular biology
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Heterologous Gene Expression in E.coli - Methods and Protocols (Hardcover, 2011)
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Heterologous Gene Expression in E.coli - Methods and Protocols (Hardcover, 2011)
Series: Methods in Molecular Biology, 705
Expected to ship within 12 - 17 working days
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Total price: R4,588
Discovery Miles: 45 880
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Protein expression in a heterologous host is a cornerstone of
biomedical research and of the biotechnology industry. Despite the
advanced state of protein expression technology improvements are
still needed. For example, membrane proteins constitute a
significant percentage of the total cellular proteins but as a
class are very difficult to overexpress, especially in a
heterologous host. The ideal host would have the ability to express
any protein, with relevant post-translational modifications, and be
as easy to work with as E. coli. In Heterologous Gene Expression in
E. coli: Methods and Protocols, expert scientists intimately
familiar with the relevant techniques offer chapters that greatly
expand the utility of this expression host. The contributions in
this detailed volume describe methods, for example, to successfully
express proteins in E. coli that would otherwise form aggregates in
this host, to add post-translational modifications, to incorporate
non-standard amino acid residues or moieties into E. coli expressed
proteins, to identify binding partners, and to express membrane
proteins. Written in the highly successful Methods in Molecular
Biology(TM) format, chapters include introductions to their
respective subjects, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Practical and
cutting-edge, Heterologous Gene Expression in E. coli: Methods and
Protocols seeks to familiarize the researcher with the myriad of E.
coli expression strains available and move E. coli closer to that
ideal of the perfect host.
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